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Home > Products > N0702-At488-S

anti-Chicken IgY sdAb - FluoTag-X2 - N0702-At488-S

Secondary single domain antibody specific for chicken IgY

 

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N0702-At488-S
Amount: 500 µl
Price: $250.00
Cat. No. N0702-At488-S 500 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488.

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used.

ATTO® 488: λex 500 nm / λem 520 nm
ATTO® 550: λex 554 nm / λem 576 nm
ATTO® 565: λex 564 nm / λem 590 nm
ATTO® 594: λex 603 nm / λem 626 nm
ATTO® 647N: λex 646 nm / λem 664 nm
 

ATTO®488, ATTO®565, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®488, ATTO®565 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH.

 Albumin was added for stabilization. For reconstitution add 500 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect form light!
Applications
 
ICC: 1 : 500
IHC: 1 : 500
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 4B1
Subtype single domain
Reactivity Reacts with: chicken.
No signal: mouse, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
Specificity Recognizes native chicken IgY. It does not cross-react to mouse, rabbit, Guinea pig, rat, donkey, goat, pig, horse, cow or human IgGs.

References for anti-Chicken IgY sdAb - N0702-At488-S

5-HT4R agonism reduces L-DOPA-induced dyskinesia via striatopallidal neurons in unilaterally 6-OHDA lesioned mice.
Ballardin D, Makrini-Maleville L, Seper A, Valjent E, Rebholz H
Neurobiology of disease (2024) 198: 106559. N0702-At488-S IHC; tested species: mouse

Cat. No.: N0702-At488-S
Amount: 500 µl
Price: $250.00
5-HT4R agonism reduces L-DOPA-induced dyskinesia via striatopallidal neurons in unilaterally 6-OHDA lesioned mice.
Ballardin D, Makrini-Maleville L, Seper A, Valjent E, Rebholz H
Neurobiology of disease (2024) 198: 106559. N0702-At488-S IHC; tested species: mouse
Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

IgY is the major immunoglobulin found in chicken eggs. FluoTag®-X2 anti-chicken is a species-specific FluoTag®-X2 directed against immunoglobulin IgY from chicken.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary chicken antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization.
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