Cat. No.: N0502-At488-L
Amount: 200 µl
Price:
$515.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N0502-At488-L |
200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with
ATTO® 488.
Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Storage |
Up to three months: -20°C Up to 12 months: -80°C or below Protect form light! |
| Applications | |
| Label | ATTO 488, two fluorophores coupled to one FluoTag |
| Clone | 1H7 |
| Subtype | single domain |
| Immunogen | Recombinant protein corresponding to AA 1 to 232 from mTag-BFP |
| Specificity |
Recognizes mTagBFP, mTagRFP and mKate and its most common fluorescent proteins deriving from Entacmaea quadricolor. Does not cross-react with common GFP- or dsRed derivatives. |
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Blue fluorescent protein BFP is a monomeric fast maturating and highly photostable protein that is suited for protein labeling in acidic environments due to its high pH stability. BFP has a bright blue fluorescence with excitation/emission maxima at 402 and 457 nm and is a preferred candidate for multi color imaging.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-Q each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a coupling efficiency of > 95% guarantees a highly linear relation between the number of target molecules and the intensity of fluorescence. This enables a direct count of the target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
In FluoTag®-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets up to four fluorophores (in X4 variants) to the protein of interest, which ensures extra-bright signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.