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Home > Products > 363 003

N-Cadherin antibody - 363 003

N-Cadherin is a cell adhesion protein
Rabbit polyclonal purified antibody
Cat. No.: 363 003
Amount: 50 µg
Price: $375.00
Cat. No. 363 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 746 to 906 from mouse N-Cadherin (UniProt Id: P15116)
Reactivity Reacts with: rat (Q9Z1Y3), mouse (P15116).
Other species not tested yet.
Specificity Recognizes N-cadherin. The antibody may crossreact to other cadherins due to sequence homology.
Remarks

IHC: Fix for 15 min with 4% PFA and 15% picric acid in PBS.
Data sheet 363_003.pdf

References for N-Cadherin - 363 003

Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects.
Benavides-Rivas C, Tovar LM, Zúñiga N, Pinto-Borguero I, Retamal C, Yévenes GE, Moraga-Cid G, Fuentealba J, Guzmán L, Coddou C, Bascuñán-Godoy L, et al.
Frontiers in pharmacology (2020) 11: 900. 363 003 WB; tested species: frog
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 363 003 IHC; tested species: rat
Choroid plexus epithelial cells express the adhesion protein P-cadherin at cell-cell contacts and syntaxin-4 in the luminal membrane domain.
Christensen IB, Mogensen EN, Damkier HH, Praetorius J
American journal of physiology. Cell physiology (2018) 3145: C519-C533. 363 003 IHC-P; tested species: mouse
Cat. No.: 363 003
Amount: 50 µg
Price: $375.00
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects.
Benavides-Rivas C, Tovar LM, Zúñiga N, Pinto-Borguero I, Retamal C, Yévenes GE, Moraga-Cid G, Fuentealba J, Guzmán L, Coddou C, Bascuñán-Godoy L, et al.
Frontiers in pharmacology (2020) 11: 900. 363 003 WB; tested species: frog
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 363 003 IHC; tested species: rat
Choroid plexus epithelial cells express the adhesion protein P-cadherin at cell-cell contacts and syntaxin-4 in the luminal membrane domain.
Christensen IB, Mogensen EN, Damkier HH, Praetorius J
American journal of physiology. Cell physiology (2018) 3145: C519-C533. 363 003 IHC-P; tested species: mouse
WB
ICC
IHC
IHC-P
Background
N-cadherin or neural cadherin, also referred to as Cadherin 2, CDH 2 and NCAD, is a transmembrane protein that functions in calcium-dependent cell-cell adhesion. The extracellular region interacts with another N-cadherin molecule on an adjacent cell in an anti-parallel conformation. A repeated set of dimer interfaces forms a linear, adhesive "zipper" between cells. The C-terminal, cytoplasmic tail mediates binding to catenins, which in turn interact with the actin cytoskeleton.
Cleavage of N-cadherin regulates adult neural stem cell functional quiescence.