Synaptic Systems - Munc18-2

Cat. No.: 116 102

Amount: 200 µl

Price: $355.00

Cat. No. 116 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 593 from mouse Munc18-2 (UniProt Id: Q64324)
Reactivity	Reacts with: rat (Q62753), mouse (Q64324). Other species not tested yet.
Specificity	Specific for Munc 18-2 with weak cross-reactivity to Munc18-1 and 3.
Remarks	This antibody detects two smaller bands (possible degradation products) of unkown identity.
Data sheet	116_102.pdf

References for Munc18-2 - 116 102

Munc18-1 is essential for neuropeptide secretion in neurons.
Puntman DC, Arora S, Farina M, Toonen RF, Verhage M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 116 102 WB; tested species: mouse

Platelet-specific deletion of SNAP23 ablates granule secretion, substantially inhibiting arterial and venous thrombosis in mice.
Williams CM, Li Y, Brown E, Poole AW
Blood advances (2018) 224: 3627-3636. 116 102 WB; tested species: mouse

Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis.
Tsai PS, Brewis IA, van Maaren J, Gadella BM
PloS one (2012) 73: e32603. 116 102 WB, ICC; tested species: pig

Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cell.
Cepeda AP, Ninov M, Neef J, Parfentev I, Kusch K, Reisinger E, Jahn R, Moser T, Urlaub H
Molecular & cellular proteomics : MCP (2023) : 100704. 116 102 IHC; tested species: mouse

Cat. No.: 116 102

Amount: 200 µl

Price: $355.00

Background

Munc 18 is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin 1. It is highly homologous to the C. elegans unc-18 gene product, and weakly related to the yeast sec1, sly1, and slp1 genes.
There are three munc 18 isoforms in mammals. Munc 18-1 or 18a, also referred to as rb-sec1, n-sec1, stxbp1 and p67, is primarily expressed in neurons. Munc 18-2 or 18b, also referred to as stxbp2, and Munc 18-3 or 18c are expressed ubiquitously.

1 ) Tellam JT et al., J. Biol. Chem. (1995)
2 ) Hata Y et al., J. Biol. Chem. (1995)
3 ) Pevsner J et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
4 ) Garcia EP et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
5 ) Hata Y et al., Nature (1993)
6 ) Fukuda M et al., J. Biol. Chem. (2005)
7 ) Martin-Verdeaux S et al., J. Cell. Sci. (2003)
8 ) Riento K et al., J. Biol. Chem. (2000)

Protocols

Munc18-2 antibody - 116 102

References for Munc18-2 - 116 102