Synaptic Systems - Munc18-1

Cat. No.: 116 004

Amount: 100 µl

Price: $365.00

Cat. No. 116 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 580 to 594 from rat Munc18-1 (UniProt Id: P61765)
Reactivity	Reacts with: rat (P61765), mouse. Other species not tested yet.
Matching control protein/peptide	116-0P
Data sheet	116_004.pdf

References for Munc18-1 - 116 004

N-methyl-D-aspartate receptor dysfunction by unmutated human antibodies against the NR1 subunit.
Wenke NK, Kreye J, Andrzejak E, van Casteren A, Leubner J, Murgueitio MS, Reincke SM, Secker C, Schmidl L, Geis C, Ackermann F, et al.
Annals of neurology (2019) 855: 771-776. 116 004 ICC; tested species: mouse

Human Autoantibodies against the AMPA Receptor Subunit GluA2 Induce Receptor Reorganization and Memory Dysfunction.
Haselmann H, Mannara F, Werner C, Planagumà J, Miguez-Cabello F, Schmidl L, Grünewald B, Petit-Pedrol M, Kirmse K, Classen J, Demir F, et al.
Neuron (2018) : . 116 004 ICC; tested species: mouse

Cat. No.: 116 004

Amount: 100 µl

Price: $365.00

ICC

IHC

IHC-P

Background

Munc 18 is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin 1. It is highly homologous to the C. elegans unc-18 gene product, and weakly related to the yeast sec1, sly1, and slp1 genes.
There are three munc 18 isoforms in mammals. Munc 18-1 or 18a, also referred to as rb-sec1, n-sec1, stxbp1 and p67, is primarily expressed in neurons. Munc 18-2 or 18b, also referred to as stxbp2, and Munc 18-3 or 18c are expressed ubiquitously.

1 ) Tellam JT et al., J. Biol. Chem. (1995)
2 ) Hata Y et al., J. Biol. Chem. (1995)
3 ) Pevsner J et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
4 ) Garcia EP et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
5 ) Hata Y et al., Nature (1993)
6 ) Fukuda M et al., J. Biol. Chem. (2005)
7 ) Martin-Verdeaux S et al., J. Cell. Sci. (2003)
8 ) Riento K et al., J. Biol. Chem. (2000)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Munc18-1 antibody - 116 004

References for Munc18-1 - 116 004