Synaptic Systems - Munc18-1

Cat. No.: 116 003

Amount: 50 µg

Price: $455.00

Cat. No. 116 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery DNA-PAINT: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Immunogen	Synthetic peptide corresponding to AA 580 to 594 from rat Munc18-1 (UniProt Id: P61765)
Reactivity	Reacts with: human (P61764), rat (P61765), mouse (O08599), cow. Other species not tested yet.
Specificity	K.D. validated PubMed: 34103363
Matching control protein/peptide	116-0P
Remarks	DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet	116_003.pdf

References for Munc18-1 - 116 003

Brain-region-specific changes in neurons and glia and dysregulation of dopamine signaling in Grin2a mutant mice.
Farsi Z, Nicolella A, Simmons SK, Aryal S, Shepard N, Brenner K, Lin S, Herzog L, Moran SP, Stalnaker KJ, Shin W, et al.
Neuron (2023) 11121: 3378-3396.e9. 116 003 WB; tested species: mouse

Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity.
Barber CN, Goldschmidt HL, Ma Q, Devine LR, Cole RN, Huganir RL, Raben DM
Frontiers in synaptic neuroscience (2022) 14: 855673. 116 003 WB; tested species: rat

Bidirectional regulation of synaptic SUMOylation by Group 1 metabotropic glutamate receptors.
Pronot M, Poupon G, Pizzamiglio L, Prieto M, Chato-Astrain I, Lacagne I, Schorova L, Folci A, Brau F, Martin S
Cellular and molecular life sciences : CMLS (2022) 797: 378. 116 003 WB; tested species: rat

Munc18-1 is essential for neuropeptide secretion in neurons.
Puntman DC, Arora S, Farina M, Toonen RF, Verhage M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 116 003 WB; KD verified; tested species: mouse

Protein instability, haploinsufficiency, and cortical hyper-excitability underlie STXBP1 encephalopathy.
Kovacevic J, Maroteaux G, Schut D, Loos M, Dubey M, Pitsch J, Remmelink E, Koopmans B, Crowley J, Cornelisse LN, Sullivan PF, et al.
Brain : a journal of neurology (2018) : . 116 003 ICC; tested species: mouse

Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 116 003 ICC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 116 003 DNA-PAINT; tested species: rat

Cat. No.: 116 003

Amount: 50 µg

Price: $455.00

Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 116 003 ICC; tested species: mouse

ICC

IHC

IHC-P

Background

Munc 18 is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin 1. It is highly homologous to the C. elegans unc-18 gene product, and weakly related to the yeast sec1, sly1, and slp1 genes.
There are three munc 18 isoforms in mammals. Munc 18-1 or 18a, also referred to as rb-sec1, n-sec1, stxbp1 and p67, is primarily expressed in neurons. Munc 18-2 or 18b, also referred to as stxbp2, and Munc 18-3 or 18c are expressed ubiquitously.

1 ) Tellam JT et al., J. Biol. Chem. (1995)
2 ) Hata Y et al., J. Biol. Chem. (1995)
3 ) Pevsner J et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
4 ) Garcia EP et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
5 ) Hata Y et al., Nature (1993)
6 ) Fukuda M et al., J. Biol. Chem. (2005)
7 ) Martin-Verdeaux S et al., J. Cell. Sci. (2003)
8 ) Riento K et al., J. Biol. Chem. (2000)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Munc18-1 antibody - 116 003 K.D.

References for Munc18-1 - 116 003