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Home > Products > 116 003

Munc18-1 antibody - 116 003 K.D.

Munc18 proteins are peripheral membrane protein involved in vesicle docking and fusion
Rabbit polyclonal purified antibody
Cat. No.: 116 003
Amount: 50 µg
Price: $455.00
Cat. No. 116 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
DNA-PAINT: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 580 to 594 from rat Munc18-1 (UniProt Id: P61765)
Reactivity Reacts with: human (P61764), rat (P61765), mouse (O08599), cow.
Other species not tested yet.
Specificity K.D. validated PubMed: 34103363
Matching control protein/peptide 116-0P
Remarks

DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet 116_003.pdf

References for Munc18-1 - 116 003

Brain-region-specific changes in neurons and glia and dysregulation of dopamine signaling in Grin2a mutant mice.
Farsi Z, Nicolella A, Simmons SK, Aryal S, Shepard N, Brenner K, Lin S, Herzog L, Moran SP, Stalnaker KJ, Shin W, et al.
Neuron (2023) 11121: 3378-3396.e9. 116 003 WB; tested species: mouse
Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity.
Barber CN, Goldschmidt HL, Ma Q, Devine LR, Cole RN, Huganir RL, Raben DM
Frontiers in synaptic neuroscience (2022) 14: 855673. 116 003 WB; tested species: rat
Bidirectional regulation of synaptic SUMOylation by Group 1 metabotropic glutamate receptors.
Pronot M, Poupon G, Pizzamiglio L, Prieto M, Chato-Astrain I, Lacagne I, Schorova L, Folci A, Brau F, Martin S
Cellular and molecular life sciences : CMLS (2022) 797: 378. 116 003 WB; tested species: rat
Munc18-1 is essential for neuropeptide secretion in neurons.
Puntman DC, Arora S, Farina M, Toonen RF, Verhage M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 116 003 WB; KD verified; tested species: mouse
Protein instability, haploinsufficiency, and cortical hyper-excitability underlie STXBP1 encephalopathy.
Kovacevic J, Maroteaux G, Schut D, Loos M, Dubey M, Pitsch J, Remmelink E, Koopmans B, Crowley J, Cornelisse LN, Sullivan PF, et al.
Brain : a journal of neurology (2018) : . 116 003 ICC; tested species: mouse
Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 116 003 ICC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 116 003 DNA-PAINT; tested species: rat
Cat. No.: 116 003
Amount: 50 µg
Price: $455.00
Brain-region-specific changes in neurons and glia and dysregulation of dopamine signaling in Grin2a mutant mice.
Farsi Z, Nicolella A, Simmons SK, Aryal S, Shepard N, Brenner K, Lin S, Herzog L, Moran SP, Stalnaker KJ, Shin W, et al.
Neuron (2023) 11121: 3378-3396.e9. 116 003 WB; tested species: mouse
Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity.
Barber CN, Goldschmidt HL, Ma Q, Devine LR, Cole RN, Huganir RL, Raben DM
Frontiers in synaptic neuroscience (2022) 14: 855673. 116 003 WB; tested species: rat
Bidirectional regulation of synaptic SUMOylation by Group 1 metabotropic glutamate receptors.
Pronot M, Poupon G, Pizzamiglio L, Prieto M, Chato-Astrain I, Lacagne I, Schorova L, Folci A, Brau F, Martin S
Cellular and molecular life sciences : CMLS (2022) 797: 378. 116 003 WB; tested species: rat
Munc18-1 is essential for neuropeptide secretion in neurons.
Puntman DC, Arora S, Farina M, Toonen RF, Verhage M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 116 003 WB; KD verified; tested species: mouse
Protein instability, haploinsufficiency, and cortical hyper-excitability underlie STXBP1 encephalopathy.
Kovacevic J, Maroteaux G, Schut D, Loos M, Dubey M, Pitsch J, Remmelink E, Koopmans B, Crowley J, Cornelisse LN, Sullivan PF, et al.
Brain : a journal of neurology (2018) : . 116 003 ICC; tested species: mouse
Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 116 003 ICC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 116 003 DNA-PAINT; tested species: rat
WB
ICC
IHC
IHC-P
Background

Munc 18 is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin 1. It is highly homologous to the C. elegans unc-18 gene product, and weakly related to the yeast sec1, sly1, and slp1 genes.
There are three munc 18 isoforms in mammals. Munc 18-1 or 18a, also referred to as rb-sec1, n-sec1, stxbp1 and p67, is primarily expressed in neurons. Munc 18-2 or 18b, also referred to as stxbp2, and Munc 18-3 or 18c are expressed ubiquitously.
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