Synaptic Systems - Munc13-2

Cat. No.: 126 203

Amount: 50 µg

Price: $375.00

Cat. No. 126 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 151 to 317 from rat Munc13-2 isoform 2 (UniProt Id: Q62769-2)
Reactivity	Reacts with: rat (Q62769), mouse (Q9Z1N9). Other species not tested yet.
Specificity	Specific for munc 13-2 brain specific isoform. K.O. validated
Remarks	IHC: This antibody requires mild fixation with 1% PFA at low pH (pH 6.0).
Data sheet	126_203.pdf

References for Munc13-2 - 126 203

Differential Expression of Munc13-2 Produces Unique Synaptic Phenotypes in the Basolateral Amygdala of C57BL/6J and DBA/2J Mice.
Gioia DA, Alexander NJ, McCool BA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3643: 10964-10977. 126 203 WB

Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 126 203 IHC; KD verified; tested species: mouse,rat

RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, et al.
eLife (2019) 8: . 126 203 IHC; tested species: mouse

Cat. No.: 126 203

Amount: 50 µg

Price: $375.00

IHC

Background

Munc 13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc 13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc 13-2 is mainly expressed in cortex and hippocampus whereas Munc 13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described.
Munc 13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.

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2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Ohtsuka T et al., J. Cell Biol. (2002)
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6 ) Betz A et al., J. Biol. Chem. (1997)
7 ) Südhof TC et al., Nature (1995)
8 ) Brose N et al., J. Biol. Chem. (1995)
9 ) Goldenberg NM et al., Am. J. Physiol., Cell Physiol. (2009)
10 ) Zhu Y et al., J. Physiol. (Lond.) (2008)

Protocols

IHC

Other products for this target

Munc13-2 antibody - 126 203 K.O.

References for Munc13-2 - 126 203