Synaptic Systems - Munc13-1

Cat. No.: 126 104

Amount: 100 µl

Price: $365.00

Cat. No. 126 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity	Reacts with: rat (Q62768), mouse (Q4KUS2), zebrafish. Other species not tested yet.
Specificity	K.O. validated
Remarks	IHC: This antibody requires mild fixation with 1% PFA (sodium acetate, pH 6,0).
Data sheet	126_104.pdf

References for Munc13-1 - 126 104

Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 126 104 WB; tested species: mouse

An opposing function of paralogs in balancing developmental synapse maturation.
Favaro PD, Huang X, Hosang L, Stodieck S, Cui L, Liu YZ, Engelhardt KA, Schmitz F, Dong Y, Löwel S, Schlüter OM, et al.
PLoS biology (2018) 1612: e2006838. 126 104 WB, IHC; tested species: mouse

Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins.
Uemura T, Suzuki-Kouyama E, Kawase S, Kurihara T, Yasumura M, Yoshida T, Fukai S, Yamazaki M, Fei P, Abe M, Watanabe M, et al.
Cell reports (2022) 391: 110624. 126 104 ICC; tested species: mouse

Disentangling the roles of RIM and Munc13 in synaptic vesicle localization and neurotransmission.
Zarebidaki F, Camacho M, Brockmann MM, Trimbuch T, Herman MA, Rosenmund C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 126 104 ICC; KD verified; tested species: mouse

Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 126 104 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 126 104 IHC; tested species: rat

Cat. No.: 126 104

Amount: 100 µl

Price: $365.00

ICC

IHC

Background

Munc 13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc 13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc 13-2 is mainly expressed in cortex and hippocampus whereas Munc 13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described.
Munc 13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.

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3 ) Ohtsuka T et al., J. Cell Biol. (2002)
4 ) Ashery U et al., EMBO J. (2000)
5 ) Brose N et al., Curr. Opin. Neurobiol. (2000)
6 ) Betz A et al., J. Biol. Chem. (1997)
7 ) Südhof TC et al., Nature (1995)
8 ) Brose N et al., J. Biol. Chem. (1995)
9 ) Goldenberg NM et al., Am. J. Physiol., Cell Physiol. (2009)
10 ) Zhu Y et al., J. Physiol. (Lond.) (2008)

Protocols

ICC

IHC

Other products for this target

Munc13-1 antibody - 126 104 K.O.

References for Munc13-1 - 126 104