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Home > Products > 126 104

Munc13-1 antibody - 126 104 K.O.

Munc13 proteins are the mammalian homologues of the C. elegans unc-13 gene essential for exocytosis
Guinea pig polyclonal antiserum
Cat. No.: 126 104
Amount: 100 µl
Price: $365.00
Cat. No. 126 104 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity Reacts with: rat (Q62768), mouse (Q4KUS2), zebrafish.
Other species not tested yet.
Specificity K.O. validated
Remarks

IHC: This antibody requires mild fixation with 1% PFA (sodium acetate, pH 6,0).
Data sheet 126_104.pdf

References for Munc13-1 - 126 104

Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 126 104 WB; tested species: mouse
An opposing function of paralogs in balancing developmental synapse maturation.
Favaro PD, Huang X, Hosang L, Stodieck S, Cui L, Liu YZ, Engelhardt KA, Schmitz F, Dong Y, Löwel S, Schlüter OM, et al.
PLoS biology (2018) 1612: e2006838. 126 104 WB, IHC; tested species: mouse
Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins.
Uemura T, Suzuki-Kouyama E, Kawase S, Kurihara T, Yasumura M, Yoshida T, Fukai S, Yamazaki M, Fei P, Abe M, Watanabe M, et al.
Cell reports (2022) 391: 110624. 126 104 ICC; tested species: mouse
Disentangling the roles of RIM and Munc13 in synaptic vesicle localization and neurotransmission.
Zarebidaki F, Camacho M, Brockmann MM, Trimbuch T, Herman MA, Rosenmund C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 126 104 ICC; KD verified; tested species: mouse
Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 126 104 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 126 104 IHC; tested species: rat
An opposing function of paralogs in balancing developmental synapse maturation.
Favaro PD, Huang X, Hosang L, Stodieck S, Cui L, Liu YZ, Engelhardt KA, Schmitz F, Dong Y, Löwel S, Schlüter OM, et al.
PLoS biology (2018) 1612: e2006838. 126 104 WB, IHC; tested species: mouse
Cat. No.: 126 104
Amount: 100 µl
Price: $365.00
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 126 104 WB; tested species: mouse
An opposing function of paralogs in balancing developmental synapse maturation.
Favaro PD, Huang X, Hosang L, Stodieck S, Cui L, Liu YZ, Engelhardt KA, Schmitz F, Dong Y, Löwel S, Schlüter OM, et al.
PLoS biology (2018) 1612: e2006838. 126 104 WB, IHC; tested species: mouse
Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins.
Uemura T, Suzuki-Kouyama E, Kawase S, Kurihara T, Yasumura M, Yoshida T, Fukai S, Yamazaki M, Fei P, Abe M, Watanabe M, et al.
Cell reports (2022) 391: 110624. 126 104 ICC; tested species: mouse
Disentangling the roles of RIM and Munc13 in synaptic vesicle localization and neurotransmission.
Zarebidaki F, Camacho M, Brockmann MM, Trimbuch T, Herman MA, Rosenmund C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 126 104 ICC; KD verified; tested species: mouse
Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 126 104 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 126 104 IHC; tested species: rat
An opposing function of paralogs in balancing developmental synapse maturation.
Favaro PD, Huang X, Hosang L, Stodieck S, Cui L, Liu YZ, Engelhardt KA, Schmitz F, Dong Y, Löwel S, Schlüter OM, et al.
PLoS biology (2018) 1612: e2006838. 126 104 WB, IHC; tested species: mouse
WB
ICC
IHC
Background
Munc 13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc 13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc 13-2 is mainly expressed in cortex and hippocampus whereas Munc 13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described.
Munc 13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.
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