MT3 antibody - 333 003

MT3 is a major regulator of cellular zinc in the brain

Rabbit polyclonal purified antibody

Cat. No.: 333 003

Amount: 50 µg

Price: $375.00

Cat. No. 333 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 68 from human MT3 (UniProt Id: P25713)
Reactivity	Reacts with: human (P25713), rat (P37361), mouse (P28184). Other species not tested yet.
Specificity	Shows band of expected size in westernblots. The band is blocked by pre-adsorption with the immunogen.
Data sheet	333_003.pdf

Cat. No.: 333 003

Amount: 50 µg

Price: $375.00

Background

Metallothioneins are cysteine rich proteins of low molecular weight that show ubiquitous expression patterns. Four members MT 1, 2, 3 and 4 have been described, so far. MT 3, also known as Zn7MT3 and GIF, is mainly expressed in the brain and exhibits only very low expression levels in other tissues where the other MTs predominate.The precise biological function of MT 3 is unknown but it is regarded as one of the major regulators of cellular zinc in the brain.

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