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MPP5 antibody - 220 003

MPP5 is a multi adaptor protein
Rabbit polyclonal purified antibody
Cat. No.: 220 003
Amount: 50 µg
Price: $375.00
Cat. No. 220 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 2 to 244 from human MPP5 (UniProt Id: Q8N3R9)
Reactivity Reacts with: human (Q8N3R9), rat, mouse (Q9JLB2), pig, cow.
Other species not tested yet.
Remarks

IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.

Data sheet 220_003.pdf
Cat. No.: 220 003
Amount: 50 µg
Price: $375.00
Background

The membrane palmitoylated protein 5 (MPP 5), also referred to as PALS 1, belongs to the membrane associated guanylate kinase (MAGUK) protein family. It is a multi adaptor protein that interacts via its PSD-95/Dlg/ZO-1 (PDZ) and L-27 domains with the proteins Crb 1, Crb 3 and Patj. It has been shown to be involved in the establishment of apicobasal cell polarity.