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Home > Products > 220 103

MPP4 antibody - 220 103

MPP4 is a multi adaptor protein
Rabbit polyclonal purified antibody
Cat. No.: 220 103
Amount: 50 µg
Price: $380.00
Cat. No. 220 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 5000 up to 1 : 20000 (see remarks) gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 197 to 216 from mouse MPP4 (UniProt Id: Q6P7F1)
Reactivity Reacts with: rat (Q9QYH1), mouse (Q6P7F1).
Other species not tested yet.
Remarks

IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.
Data sheet 220_103.pdf

References for MPP4 - 220 103

The first synapse in vision in the aging mouse retina.
Gierke K, Lux UT, Regus-Leidig H, Brandstätter JH
Frontiers in cellular neuroscience (2023) 17: 1291054. 220 103 IHC; tested species: mouse
Cat. No.: 220 103
Amount: 50 µg
Price: $380.00
The first synapse in vision in the aging mouse retina.
Gierke K, Lux UT, Regus-Leidig H, Brandstätter JH
Frontiers in cellular neuroscience (2023) 17: 1291054. 220 103 IHC; tested species: mouse
WB
IHC
Background

The membrane palmitoylated protein 4 (MPP 4), also referred to as DLG 6, belongs to the membrane associated guanylate kinase (MAGUK) protein family. It is scaffolding protein that is involved in connecting cilia and synaptic structures. It has been shown to interact with MPP 5 and Crb 1.