MOG antibody - 401 004

MOG is a component of the myelin sheath

Guinea pig polyclonal antiserum

Cat. No.: 401 004

Amount: 100 µl

Price: $365.00

Cat. No. 401 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 250 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 60 to 75 and 71 to 86 from mouse Myelin oligodendrocyte glycoprotein (UniProt Id: Q61885)
Reactivity	Reacts with: mouse (Q61885), rat (Q63345), human (Q16653). Other species not tested yet.
Remarks	IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet	401_004.pdf

Cat. No.: 401 004

Amount: 100 µl

Price: $365.00

IHC

IHC-P

Background

The myelin oligodendrocyte glycoprotein (MOG) is an integral membrane protein belonging to the immunoglobulin superfamily. It is found exclusively in the central nervous system, where it is localized at the outermost surface of myelin sheaths and oligodendrocyte membranes.
MOG is an important marker for oligodendrocyte maturation since it's expression is delayed compared with other major myelin proteins. It may function in completion, compaction, and/or maintenance of myelin, as it has an adhesive role within the CNS. Consistent with this, a homodimeric form of MOG has been observed.
MOG is a key target of demyelinating autoantibodies in inflammatory demyelinating diseases of the CNS, such as multiple sclerosis (MS).

1 ) Peschl P et al., Front Immunol (2017)
2 ) Reindl M et al., Nat Rev Neurol (2013)
3 ) Clements CS et al., Proc. Natl. Acad. Sci. U.S.A. (2003)
4 ) Johns TG et al., J. Neurochem. (1999)

Protocols

IHC

IHC-P