Cat. No.: 144-1P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 144-1P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Recombinant protein corresponding to AA 2 to 265 from rat Mint1 (UniProt Id: O35430) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 144 103 |
| Remarks |
This control protein consists of the recombinant protein (aa 2 265 of rat Mint 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 144-1p.pdf |
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Mints (also referred to as X11-like proteins) are adaptor-proteins that consist of divergent N-terminal sequences and conserved C-terminal PTB and PDZ domains.
Three isoforms (Mint 1, 2 and 3) have been described. Mint 1 exclusively binds to the synaptic protein CASK via its N-terminal sequence. Munc18-1 has been shown to be an interaction partner of Mint 1 and 2. The more C-terminal located PTB and PDZ domains present in all Mint isoforms bind to widely distributed proteins like APP, presenilins and Ca2+ channels.
A Mint 1 knock out had no obvious effect on brain achitecture and development, nor was synaptic plasticity in excitatory synapses affected. In inhibitory synapses of knock out strains the release of gamma-aminobutyric acid (GABA) was impaired.