MARK2 antibody - 331 003

MARK2 is a kinase involved in neurodegenerative diseases

Rabbit polyclonal purified antibody

Cat. No.: 331 003

Amount: 50 µg

Price: $375.00

Cat. No. 331 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 15 from mouse MARK2 (UniProt Id: Q05512)
Reactivity	Reacts with: rat (O08679), mouse (Q05512). Other species not tested yet.
Specificity	Specific for MARK 2, epitope is present in all four isoforms.
Data sheet	331_003.pdf

Cat. No.: 331 003

Amount: 50 µg

Price: $375.00

Background

The microtubule-affinity regulating kinase 2 (MARK 2), also referred to as Par-1b and EMK 1, was originally discovered by its ability to phosphorylate tau and related microtubule-associated proteins. It is involved in the regulation of many cellular processes like determination of polarity, intracellular signal transduction, transport, and cytoskeletal stability. Four isoforms are known so far.
MARK 2 has also been identified as an upstream regulator of PTEN-induced kinase 1 (PINK 1), a mitochondria-targeted serine/threonine kinase. Disbalanced functions of these proteins are connected to neurodegenerative disorders like Alzheimer's and Parkinson's disease.

1 ) Matenia D et al., Front Mol Neurosci (2014)
2 ) Gu GJ et al., J. Alzheimers Dis. (2013)
3 ) Nishimura Y et al., PLoS ONE (2012)
4 ) Matenia D et al., J. Biol. Chem. (2012)
5 ) Sapir T et al., J. Neurosci. (2008)
6 ) Sapir T et al., J. Neurosci. (2008)
7 ) Drewes G et al., Cell (1997)

Protocols