Maltose binding protein antibody - 416 003

Maltose bindin protein MBP is a part oft the MalEFGK complex

Rabbit polyclonal purified antibody

Cat. No.: 416 003

Amount: 50 µg

Price: $375.00

Cat. No. 416 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant full length maltose binding protein from E. coli. (UniProt Id: O00258)
Reactivity	Reacts with: E.coli (P0AEX9). Other species not tested yet.
Data sheet	416_003.pdf

Cat. No.: 416 003

Amount: 50 µg

Price: $375.00

Background

The maltose/maltodextrin-binding periplasmic protein is part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. It binds maltose and higher maltodextrins such as maltotriose or amylose and is therefore used as a protein-tag for affinitypurification of recombinant proteins.

1 ) Kellermann O et al., Eur. J. Biochem. (1974)
2 ) Szmelcman S et al., Eur. J. Biochem. (1976)
3 ) Davidson AL et al., J. Biol. Chem. (1990)
4 ) Davidson AL et al., J. Biol. Chem. (1991)
5 ) Maina CV et al., Gene (1988)

Protocols

Standard protocol