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m6A antibody - 202 011

N6-methyladenosine or m6A are posttranscritional modifications of RNA and DNA
Mouse monoclonal purified IgG
Cat. No.: 202 011
Amount: 100 µg
Price: $415.00
Cat. No. 202 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: (AP staining) suitable for WB and Dot Blot
Dot blot: 1 : 1000 gallery  
IP: yes
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
MeRIP: yes
Clone 345E11
Subtype IgG2b (κ light chain)
Immunogen N6-methyladenosine fused to BSA.
Reactivity Reacts with: human, rat, mouse, eukaryotes, prokaryotes.
Other species not tested yet.
Specificity Specific for N6-methyladenosine (m6A) with some cross-reactivity to m6Am.
Data sheet 202_011.pdf

References for m6A - 202 011

N6-methyladenosine marks primary microRNAs for processing.
Alarcón CR, Lee H, Goodarzi H, Halberg N, Tavazoie SF
Nature (2015) 5197544: 482-5. 202 011 WB
Erasing m6A-dependent transcription signature of stress-sensitive genes triggers antidepressant actions.
Wu PF, Han QQ, Chen FF, Shen TT, Li YH, Cao Y, Chen JG, Wang F
Neurobiology of stress (2021) 15: 100390. 202 011 Dot blot, ICC; tested species: mouse
Temporal Control of Mammalian Cortical Neurogenesis by m6A Methylation.
Yoon KJ, Ringeling FR, Vissers C, Jacob F, Pokrass M, Jimenez-Cyrus D, Su Y, Kim NS, Zhu Y, Zheng L, Kim S, et al.
Cell (2017) 1714: 877-889.e17. 202 011 Dot blot; tested species: mouse
Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.
Linder B, Grozhik AV, Olarerin-George AO, Meydan C, Mason CE, Jaffrey SR
Nature methods (2015) 128: 767-72. 202 011 Dot blot, MeRIP; tested species: human,mouse
Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation.
Koziol MJ, Bradshaw CR, Allen GE, Costa AS, Frezza C
Bio-protocol (2016) 621: . 202 011 IP
Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.
Koziol MJ, Bradshaw CR, Allen GE, Costa ASH, Frezza C, Gurdon JB
Nature structural & molecular biology (2016) 231: 24-30. 202 011 IP
Erasing m6A-dependent transcription signature of stress-sensitive genes triggers antidepressant actions.
Wu PF, Han QQ, Chen FF, Shen TT, Li YH, Cao Y, Chen JG, Wang F
Neurobiology of stress (2021) 15: 100390. 202 011 Dot blot, ICC; tested species: mouse
Role of 6mA in the Regulation of Metabolic Biosynthesis in Sorghum Callus.
Tian K, Liu C, Cai Y, Zhou C
Journal of agricultural and food chemistry (2024) 7234: 19232-19245. 202 011 MeRIP; tested species: human
Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer.
Einstein JM, Perelis M, Chaim IA, Meena JK, Nussbacher JK, Tankka AT, Yee BA, Li H, Madrigal AA, Neill NJ, Shankar A, et al.
Molecular cell (2021) 8115: 3048-3064.e9. 202 011 MeRIP; tested species: human
The topologies of N6 -Adenosine methylation (m6 A) in land plant mitochondria and their putative effects on organellar gene-expression.
Murik O, Chandran SA, Nevo-Dinur K, Sultan LD, Best C, Stein Y, Hazan C, Ostersetzer-Biran O
The Plant journal : for cell and molecular biology (2019) : . 202 011 MeRIP
Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.
Linder B, Grozhik AV, Olarerin-George AO, Meydan C, Mason CE, Jaffrey SR
Nature methods (2015) 128: 767-72. 202 011 Dot blot, MeRIP; tested species: human,mouse
Cat. No.: 202 011
Amount: 100 µg
Price: $415.00
N6-methyladenosine marks primary microRNAs for processing.
Alarcón CR, Lee H, Goodarzi H, Halberg N, Tavazoie SF
Nature (2015) 5197544: 482-5. 202 011 WB
Erasing m6A-dependent transcription signature of stress-sensitive genes triggers antidepressant actions.
Wu PF, Han QQ, Chen FF, Shen TT, Li YH, Cao Y, Chen JG, Wang F
Neurobiology of stress (2021) 15: 100390. 202 011 Dot blot, ICC; tested species: mouse
Temporal Control of Mammalian Cortical Neurogenesis by m6A Methylation.
Yoon KJ, Ringeling FR, Vissers C, Jacob F, Pokrass M, Jimenez-Cyrus D, Su Y, Kim NS, Zhu Y, Zheng L, Kim S, et al.
Cell (2017) 1714: 877-889.e17. 202 011 Dot blot; tested species: mouse
Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.
Linder B, Grozhik AV, Olarerin-George AO, Meydan C, Mason CE, Jaffrey SR
Nature methods (2015) 128: 767-72. 202 011 Dot blot, MeRIP; tested species: human,mouse
Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation.
Koziol MJ, Bradshaw CR, Allen GE, Costa AS, Frezza C
Bio-protocol (2016) 621: . 202 011 IP
Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.
Koziol MJ, Bradshaw CR, Allen GE, Costa ASH, Frezza C, Gurdon JB
Nature structural & molecular biology (2016) 231: 24-30. 202 011 IP
Erasing m6A-dependent transcription signature of stress-sensitive genes triggers antidepressant actions.
Wu PF, Han QQ, Chen FF, Shen TT, Li YH, Cao Y, Chen JG, Wang F
Neurobiology of stress (2021) 15: 100390. 202 011 Dot blot, ICC; tested species: mouse
Role of 6mA in the Regulation of Metabolic Biosynthesis in Sorghum Callus.
Tian K, Liu C, Cai Y, Zhou C
Journal of agricultural and food chemistry (2024) 7234: 19232-19245. 202 011 MeRIP; tested species: human
Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer.
Einstein JM, Perelis M, Chaim IA, Meena JK, Nussbacher JK, Tankka AT, Yee BA, Li H, Madrigal AA, Neill NJ, Shankar A, et al.
Molecular cell (2021) 8115: 3048-3064.e9. 202 011 MeRIP; tested species: human
The topologies of N6 -Adenosine methylation (m6 A) in land plant mitochondria and their putative effects on organellar gene-expression.
Murik O, Chandran SA, Nevo-Dinur K, Sultan LD, Best C, Stein Y, Hazan C, Ostersetzer-Biran O
The Plant journal : for cell and molecular biology (2019) : . 202 011 MeRIP
Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.
Linder B, Grozhik AV, Olarerin-George AO, Meydan C, Mason CE, Jaffrey SR
Nature methods (2015) 128: 767-72. 202 011 Dot blot, MeRIP; tested species: human,mouse
Dot blot
Background
m6A (N6-methyladenosine) is a posttranscriptional RNA-modification found throughout all kingdoms, e.g. in vertebrate snRNAs U2, U4, U6, in viral and eukaryotic mRNAs, and in E. coli 16S rRNA.
Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. The so-called RNA methylome probably plays an important role in in the regulation of gene expression.
In E. coli Dam methylase introduces m6A modifications on the DNA level at the 5'-GATC-3' motif. This allows the cell to differentiate between the parental and the daughter strand during mismatch repair.
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