M6a Glycoprotein antibody - 238 005

M6a Glycoprotein is a neuron specific PLP family member

Guinea pig polyclonal purified antibody

Cat. No.: 238 005

Amount: 50 µg

Price: $460.00

Cat. No. 238 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 261 to 270 from rat M6a Glycoprotein (UniProt Id: Q812E9)
Reactivity	Reacts with: human (P51674), rat (Q812E9), mouse (P35802), pig, dog. Other species not tested yet.
Matching control protein/peptide	238-0P
Data sheet	238_005.pdf

Cat. No.: 238 005

Amount: 50 µg

Price: $460.00

ICC

IHC

Background

M6a and M6b are transmembrane glycoproteins with four membrane spanning domains that belong to the myelin proteolipid protein (PLP) family. In contrast to other PLP proteins that are expressed by glia cells, M6a is found only in neurons where it is concentrated at presynaptic glutamateric terminals.
M6a has been suggested to be a potential mediator of cell-cell interactions involved in axonfasciculation during development.

1 ) Cooper B et al., PLoS ONE (2009)
2 ) Cooper B et al., Brain Res. (2008)
3 ) Alfonso J et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
4 ) Roussel G et al., J. Neurocytol. (1998)
5 ) Yan Y et al., Neuron (1993)
6 ) Baumrind NL et al., Dev. Dyn. (1992)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

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