Synaptic Systems - m-AChR-2

Cat. No.: 223 017

Amount: 100 µg

Price: $415.00

Cat. No. 223 017	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 100 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	2-1-155
Subtype	IgG1
Immunogen	207 to 388 from human m-AChR-2 (UniProt Id: P08172)
Epitop	AA 207 to 388 from human m-AChR-2 (UniProt Id: P08172)
Reactivity	Reacts with: human (P08172), rat (P10980), mouse (Q9ERZ4). Other species not tested yet.
Remarks	WB: This antibody detects also higher molecular weight bands, depending on the glycosilation state of the protein.The protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	223_017.pdf

References for m-AChR-2 - 223 017

Evaluation of muscarinic agonist-induced analgesia in muscarinic acetylcholine receptor knockout mice.
Duttaroy A, Gomeza J, Gan JW, Siddiqui N, Basile AS, Harman WD, Smith PL, Felder CC, Levey AI, Wess J
Molecular pharmacology (2002) 625: 1084-93. 223 017 IHC

Subcellular redistribution of m2 muscarinic acetylcholine receptors in striatal interneurons in vivo after acute cholinergic stimulation.
Bernard V, Laribi O, Levey AI, Bloch B
The Journal of neuroscience : the official journal of the Society for Neuroscience (1998) 1823: 10207-18. 223 017 IHC

Light and electron microscopic study of m2 muscarinic acetylcholine receptor in the basal forebrain of the rat.
Levey AI, Edmunds SM, Hersch SM, Wiley RG, Heilman CJ
The Journal of comparative neurology (1995) 3513: 339-56. 223 017 IHC

Cat. No.: 223 017

Amount: 100 µg

Price: $415.00

IHC

Background

The muscarinic acetylcholine receptors comprise 5 members (m1 - m5) and mediate many acetylcholine driven cellular actions such as adenylate cyclase inhibition, phosphoinositide degeneration and potassium channel mediation. They belong to a larger family of G protein-coupled receptors. Muscarinic acetylcholine receptor 2 has been suggested to function as a presynaptic autoreceptor that inhibits acetylcholine release in the basal forebrain. It is also expressed in cardiac tissue where it is involved in mediation of bradycardia and a decrease in cardiac contractility.

1 ) Volpicelli-Daley LA et al., Mol. Pharmacol. (2003)
2 ) Zhang W et al., J. Neurosci. (2002)
3 ) Mrzljak L et al., Proc. Natl. Acad. Sci. U.S.A. (1993)

Protocols

IHC

m-AChR-2 antibody - 223 017

References for m-AChR-2 - 223 017