LRRTM4 antibody - 353 003

LRRTM4 is involved in synapse development

Rabbit polyclonal purified antibody

Cat. No.: 353 003

Amount: 50 µg

Price: $375.00

Cat. No. 353 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet ExM: 1 : 200 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Recombinant protein corresponding to AA 446 to 517 from mouse LRRTM4 (UniProt Id: Q80XG9)
Reactivity	Reacts with: rat (B4F7C5), mouse (Q80XG9). Other species not tested yet.
Remarks	ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
Data sheet	353_003.pdf

Cat. No.: 353 003

Amount: 50 µg

Price: $375.00

ExM

Background

The leucine rich repeat transmembrane (LRRTM) neuronal protein family comprises four members (LRRTM 1-4). All exhibit synaptogenic activity and localize to post-synaptic sites of excitatory synapses.
LRRTM 4 is restricted to certain brain regions with highest expression in the dentate gyrus, while LRRTM 1 and 2 show a broad expression spectrum.

1 ) Siddiqui TJ et al., Neuron (2013)
2 ) Ko J et al., J. Cell Biol. (2011)
3 ) Brose N et al., Neuron (2009)
4 ) Linhoff MW et al., Neuron (2009)
5 ) Laurén J et al., Genomics (2003)

Protocols