Ig lambda light chain antibody - 349 017

Ig lambda light chain is a useful marker for the classification of B-cell lymphoma

Rat monoclonal purified IgG

Cat. No.: 349 017

Amount: 100 µg

Price: $415.00

Cat. No. 349 017	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) IP: yes ICC: not tested yet IHC: not tested yet IHC-P: 1 : 50 up to 1 : 200 gallery FACS: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Clone	L22.18.2
Subtype	IgG2b
Immunogen	Purified light chains from mouse IgGs
Reactivity	Reacts with: mouse. Other species not tested yet.
Specificity	Specific for λ1-light chain of mouse immunoglobulins.
Data sheet	349_017.pdf

References for Ig lambda light chain - 349 017

Alternative mechanisms of receptor editing in autoreactive B cells.
Kalinina O, Doyle-Cooper CM, Miksanek J, Meng W, Prak EL, Weigert MG
Proceedings of the National Academy of Sciences of the United States of America (2011) 10817: 7125-30. 349 017 FACS; tested species: mouse

Mice completely suppressed for the expression of immunoglobulin kappa light chain.
Weiss S, Lehmann K, Raschke WC, Cohn M
Proceedings of the National Academy of Sciences of the United States of America (1984) 811: 211-5. 349 017 ; tested species: mouse

Monoclonal antibodies to murine immunoglobulin isotypes.
Weiss S, Lehmann K, Cohn M
Hybridoma (1983) 21: 49-54. 349 017 ; tested species: mouse

Cat. No.: 349 017

Amount: 100 µg

Price: $415.00

Monoclonal antibodies to murine immunoglobulin isotypes.
Weiss S, Lehmann K, Cohn M
Hybridoma (1983) 21: 49-54. 349 017 ; tested species: mouse

IHC-P

Background

Immunoglobulin (Ig) molecules consist of heavy chains and light chains of either lambda (λ) or kappa (κ) type, with κ light chains being the more common form. Immunoglobulines of the IgG, IgD and IgE type are composed of two heavy and two light chains. IgA occurs as dimeric and IgM as pentameric Ig complexes.

1 ) Westermark P et al., Ups. J. Med. Sci. (2012)

Protocols

Standard protocol

IHC-P

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