Synaptic Systems

Cat. No.: 368 103

Amount: 50 µg

Price: $375.00

Cat. No. 368 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 16 to 37 from mouse Kv7.2 (UniProt Id: Q9Z351-1)
Reactivity	Reacts with: rat (O88943), mouse (Q9Z351). Other species not tested yet.
Matching control protein/peptide	368-1P
Data sheet	368_103.pdf

References for Kv7.2 - 368 103

Ultrastructural anatomy of nodes of Ranvier in the peripheral nervous system as revealed by STED microscopy.
D'Este E, Kamin D, Balzarotti F, Hell SW
Proceedings of the National Academy of Sciences of the United States of America (2017) 1142: E191-E199. 368 103 ICC; tested species: mouse

Molecular and functional profiling of cell diversity and identity in the lateral superior olive, an auditory brainstem center with ascending and descending projections.
Maraslioglu-Sperber A, Pizzi E, Fisch JO, Kattler K, Ritter T, Friauf E
Frontiers in cellular neuroscience (2024) 18: 1354520. 368 103 IHC; tested species: mouse

Neuron-oligodendrocyte potassium shuttling at nodes of Ranvier protects against inflammatory demyelination.
Kapell H, Fazio L, Dyckow J, Schwarz S, Cruz-Herranz A, Mayer C, Campos J, D'Este E, Möbius W, Cordano C, Pröbstel AK, et al.
The Journal of clinical investigation (2023) 1337: . 368 103 EM; tested species: mouse

Cat. No.: 368 103

Amount: 50 µg

Price: $375.00

ICC

IHC

IHC-P

Background

Five voltage gated potassium channels Kv7, encoded by the genes KCNQ 1-5, have been described in mammals so far. They are integral membrane proteins and show distinct distribution patterns. Kv7.2, Kv7.3 and Kv7.5 are widely expressed in different brain regions whereas Kv7.1 and Kv7.4 show more restricted expression profiles.
Kv7.3 forms the M channel with either Kv7.2 or Kv7.5. This ion channel is a slowly activating and deactivating potassium channel that plays a critical role in the regulation of neuronal excitability.

1 ) Stewart AP et al., J. Biol. Chem. (2012)
2 ) Cooper EC et al., Proc. Natl. Acad. Sci. U.S.A. (2000)
3 ) Selyanko AA et al., J. Neurosci. (1999)
4 ) Singh NA et al., Nat. Genet. (1998)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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Kv7.2 antibody - 368 103

References for Kv7.2 - 368 103