Synaptic Systems

Cat. No.: 231-1P

Amount: 100 µg

Price: $105.00

Background

Voltage-gated potassium (Kv) channels regulate many aspects of neuronal excitability like shaping of action potentials or modulating of spike patterns.
Mammalian neurons express more than 20 different Kv subunits that can be subdivided into 12 families. Heteromeric assembly of four subunits and differential phosphorylation of Kv channels give rise to a huge molecular and functional diversity.
The Kv 2 subfamily comprising Kv 2.1 (DRK 1, Kcnb 1) and Kv 2.2 (CDRK, Kcnb 2) is a unique exception since they do not form heterodimers. Kv 2.1 is found in large clusters on the soma and proximal dendrites of pyramidal neurons.

1 ) Kihira Y et al., J. Biol. Chem. (2010)
2 ) Hermanstyne TO et al., J. Comp. Neurol. (2010)
3 ) Wray D et al., Eur. Biophys. J. (2009)
4 ) Mulholland PJ et al., J. Neurosci. (2008)
5 ) Wolf-Goldberg T et al., Mol. Pharmacol. (2006)
6 ) Kobrinsky E et al., J. Biol. Chem. (2006)
7 ) O'Connell KM et al., J. Neurosci. (2006)
8 ) Park KS et al., Science (2006)
9 ) Lim ST et al., Neuron (2000)
10 ) Murakoshi H et al., Mol. Pharmacol. (1997)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 231-1P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 859 to 873 from rat Kv2.2 (UniProt Id: Q63099)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	231 103
Remarks	This control protein consists of the synthetic peptide (aa 859-873 of mouse Kv 2.2) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	231-1p.pdf

Kv2.2 peptide - 231-1P