Synaptic Systems

Cat. No.: 231-0P

Amount: 100 µg

Price: $105.00

Background

Voltage-gated potassium (Kv) channels regulate many aspects of neuronal excitability like shaping of action potentials or modulating of spike patterns.
Mammalian neurons express more than 20 different Kv subunits that can be subdivided into 12 families. Heteromeric assembly of four subunits and differential phosphorylation of Kv channels give rise to a huge molecular and functional diversity.
The Kv 2 subfamily comprising Kv 2.1 (DRK 1, Kcnb 1) and Kv 2.2 (CDRK, Kcnb 2) is a unique exception since they do not form heterodimers. Kv 2.1 is found in large clusters on the soma and proximal dendrites of pyramidal neurons.

1 ) Kihira Y et al., J. Biol. Chem. (2010)
2 ) Hermanstyne TO et al., J. Comp. Neurol. (2010)
3 ) Wray D et al., Eur. Biophys. J. (2009)
4 ) Mulholland PJ et al., J. Neurosci. (2008)
5 ) Wolf-Goldberg T et al., Mol. Pharmacol. (2006)
6 ) Kobrinsky E et al., J. Biol. Chem. (2006)
7 ) O'Connell KM et al., J. Neurosci. (2006)
8 ) Park KS et al., Science (2006)
9 ) Lim ST et al., Neuron (2000)
10 ) Murakoshi H et al., Mol. Pharmacol. (1997)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 231-0P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 532 to 857 from rat Kv2.1 (UniProt Id: P15387)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	231 002
Remarks	This control protein consists of the recombinant mouse Kv 2.1 (aa 532 - 857) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	231-0p.pdf

Kv2.1 protein - 231-0P