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KCC2 antibody - 393 004

KCC2 is an electroneutral potassium-chloride co-transporter
Guinea pig polyclonal antiserum
Cat. No.: 393 004
Amount: 100 µl
Price: $365.00
Cat. No. 393 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 932 to 1043 from rat KCC2 (UniProt Id: Q63633)
Reactivity Reacts with: mouse, rat (Q63633).
Other species not tested yet.
Specificity Recognizes KCC 2a and KCC 2b.
Data sheet 393_004.pdf
Cat. No.: 393 004
Amount: 100 µl
Price: $365.00
Background
KCC 2 is a potassium-chloride co-transporter that is exclusively expressed in neurons of the central nervous system. The electroneutral potassium-chloride co-transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. KCC 2 is important for Cl- homeostasis in neurons.
Two isoforms, KCC 2a and KCC 2b, have been described, so far.