Synaptic Systems

Cat. No.: 180-0P

Amount: 100 µg

Price: $105.00

Background

Ionotropic glutamate receptors (iGluRs) mediate rapid excitatory neurotransmission in the mammalian CNS. They can be subdivided into three major groups, the AMPA/GluA, NMDA/GluN, and kainate/GluK receptors (KARs).
mRNAs coding for glutamate receptors are substrates for an adenosine deaminase acting on RNA (ADAR) that increases the diversity of these proteins. KARs can be found at pre- and postsynaptic sites and are composed of five different subunits: GluK1, GluK2 and GluK3 can form homomeric receptors whereas GluK4 and GluK5 form heteromeric receptors with GluK1-3.

1 ) Traynelis SF et al., Pharmacol Rev (2010)
2 ) Collingridge GL et al., Neuropharmacology (2009)
3 ) Bass BL et al., RNA (1997)
4 ) Kumar J et al., Neuron (2011)
5 ) Pinheiro PS et al., Proc. Natl. Acad. Sci. U.S.A. (2007)
6 ) Jaskolski F et al., J. Biol. Chem. (2005)
7 ) Hamassaki-Britto DE et al., J. Neurosci. (1993)
8 ) Strutz-Seebohm N et al., Cell. Physiol. Biochem. (2006)
9 ) Coussen F et al., Neuron (2005)
10 ) Ullal G et al., Epilepsia (2005)

Protocols

OTHER

Preadsorbtion

Other products for this target

Cat. No. 180-0P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 844 to 908 from rat GluK2 (UniProt Id: P42260)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	180 003
Remarks	This control protein consists of the recombinant protein (aa 844 - 908 of rat GluK 2) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	180-0p.pdf

GluK2 protein - 180-0P