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Home > Products > 277 011

Jmjd6 antibody - 277 011 K.O.

Jmjd6 is a 2-oxoglutarate-dependent oxygenase
Mouse monoclonal purified IgG
Cat. No.: 277 011
Amount: 100 µg
Price: $415.00
Cat. No. 277 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Clone mAb328
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 403 from human Jmjd6 (UniProt Id: Q6NYC1)
Reactivity Reacts with: human (Q6NYC1), rat (Q6AYK2), mouse (Q9ERI5).
Other species not tested yet.
Specificity K.O. validated PubMed: 26531897
Remarks

ICC: 0.3-0.5 % tritonx-100 are recommended for permeabilization to allow efficient penetration of the antibody.
Data sheet 277_011.pdf

References for Jmjd6 - 277 011

JMJD6 modulates DNA damage response through downregulating H4K16ac independently of its enzymatic activity.
Huo D, Chen H, Cheng Y, Song X, Zhang K, Li MJ, Xuan C
Cell death and differentiation (2019) : . 277 011 ICC; tested species: human
Intronic regulation of Aire expression by Jmjd6 for self-tolerance induction in the thymus.
Yanagihara T, Sanematsu F, Sato T, Uruno T, Duan X, Tomino T, Harada Y, Watanabe M, Wang Y, Tanaka Y, Nakanishi Y, et al.
Nature communications (2015) 6: 8820. 277 011 ICC; KO verified
Analysis of Jmjd6 cellular localization and testing for its involvement in histone demethylation.
Hahn P, Wegener I, Burrells A, Böse J, Wolf A, Erck C, Butler D, Schofield CJ, Böttger A, Lengeling A
PloS one (2010) 510: e13769. 277 011
Cat. No.: 277 011
Amount: 100 µg
Price: $415.00
JMJD6 modulates DNA damage response through downregulating H4K16ac independently of its enzymatic activity.
Huo D, Chen H, Cheng Y, Song X, Zhang K, Li MJ, Xuan C
Cell death and differentiation (2019) : . 277 011 ICC; tested species: human
Intronic regulation of Aire expression by Jmjd6 for self-tolerance induction in the thymus.
Yanagihara T, Sanematsu F, Sato T, Uruno T, Duan X, Tomino T, Harada Y, Watanabe M, Wang Y, Tanaka Y, Nakanishi Y, et al.
Nature communications (2015) 6: 8820. 277 011 ICC; KO verified
Analysis of Jmjd6 cellular localization and testing for its involvement in histone demethylation.
Hahn P, Wegener I, Burrells A, Böse J, Wolf A, Erck C, Butler D, Schofield CJ, Böttger A, Lengeling A
PloS one (2010) 510: e13769. 277 011
ICC
Background
The nuclear protein Jmjd 6 belongs to the family Jumonji domain containing proteins and catalyses the lysyl-hydroxylation of the splicing factor U2AF65. This influences alternative splicing of several endogenous and reporter genes.
In addition it has been shown to mediate the lysyl-hydroxylation of histones suggesting an implication in epigenetic regulation of gene expression or chromosomal rearangements.