Synaptic Systems

Cat. No.: 277 011

Amount: 100 µg

Price: $415.00

Cat. No. 277 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	mAb328
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 403 from human Jmjd6 (UniProt Id: Q6NYC1)
Reactivity	Reacts with: human (Q6NYC1), rat (Q6AYK2), mouse (Q9ERI5). Other species not tested yet.
Specificity	K.O. validated PubMed: 26531897
Remarks	ICC: 0.3-0.5 % tritonx-100 are recommended for permeabilization to allow efficient penetration of the antibody.
Data sheet	277_011.pdf

References for Jmjd6 - 277 011

JMJD6 modulates DNA damage response through downregulating H4K16ac independently of its enzymatic activity.
Huo D, Chen H, Cheng Y, Song X, Zhang K, Li MJ, Xuan C
Cell death and differentiation (2019) : . 277 011 ICC; tested species: human

Intronic regulation of Aire expression by Jmjd6 for self-tolerance induction in the thymus.
Yanagihara T, Sanematsu F, Sato T, Uruno T, Duan X, Tomino T, Harada Y, Watanabe M, Wang Y, Tanaka Y, Nakanishi Y, et al.
Nature communications (2015) 6: 8820. 277 011 ICC; KO verified

Analysis of Jmjd6 cellular localization and testing for its involvement in histone demethylation.
Hahn P, Wegener I, Burrells A, Böse J, Wolf A, Erck C, Butler D, Schofield CJ, Böttger A, Lengeling A
PloS one (2010) 510: e13769. 277 011

Cat. No.: 277 011

Amount: 100 µg

Price: $415.00

ICC

Background

The nuclear protein Jmjd 6 belongs to the family Jumonji domain containing proteins and catalyses the lysyl-hydroxylation of the splicing factor U2AF65. This influences alternative splicing of several endogenous and reporter genes.
In addition it has been shown to mediate the lysyl-hydroxylation of histones suggesting an implication in epigenetic regulation of gene expression or chromosomal rearangements.

1 ) Zhang J et al., Tumour Biol. (2013)
2 ) Unoki M et al., J. Biol. Chem. (2013)
3 ) Lee YF et al., Breast Cancer Res. (2012)
4 ) Mantri M et al., Chembiochem (2011)
5 ) Boeckel JN et al., Proc. Natl. Acad. Sci. U.S.A. (2011)
6 ) Webby CJ et al., Science (2009)
7 ) Chang B et al., Science (2007)

Protocols

ICC

Jmjd6 antibody - 277 011 K.O.

References for Jmjd6 - 277 011