Synaptic Systems

Cat. No.: 406 003

Amount: 50 µg

Price: $375.00

Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors.
Schoepf CL, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer KK, Kress M
Brain sciences (2020) 106: . 406 003 ICC; tested species: human,mouse

IHC

IHC-P

Background

Islet-1 (Isl1) is a member of the LIM/homeodomain family of transcription factors. It binds and regulates the promoters of the insulin, glucagon and somatostatin genes, and may play an important role in regulating insulin gene expression. It is central to the embryogenesis of pancreatic islets of Langerhans and is involved in the specification of motor neurons. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, and in inner nuclear and ganglion cell layers in the retina.
Isl1 is essential for heart development, as its expression defines cardiac progenitor cell populations and is required for normal cardiac development and asymmetry.
Mutations in this gene have been associated with maturity-onset diabetes of the young.

1 ) Khattar P et al., Stem Cells Dev. (2011)
2 ) Bu L et al., Nature (2009)
3 ) Elshatory Y et al., J. Comp. Neurol. (2007)
4 ) Elshatory Y et al., J. Neurosci. (2007)
5 ) Moretti A et al., Cell (2006)
6 ) Riggs AC et al., Diabetes (1995)

Protocols

IHC

IHC-P

Cat. No. 406 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 100 gallery IHC-P: 1 : 200 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 333 to 349 from mouse Islet1 (UniProt Id: P61372)
Reactivity	Reacts with: mouse (P61372), rat (P61374). Other species not tested yet.
Specificity	The antibody may crossreact with Islet-2 due to sequence homology.
Data sheet	406_003.pdf

Islet-1 antibody - 406 003

References for Islet-1 - 406 003