Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 117 003

IP3-receptor type1 antibody - 117 003

IP3-receptor triggers the release of Ca2+ from intracellular stores
Rabbit polyclonal purified antibody
Cat. No.: 117 003
Amount: 50 µg
Price: $455.00
Cat. No. 117 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 200 gallery  
Immunogen Synthetic peptide corresponding to AA 2731 to 2749 from rat IP3-receptortype1 (UniProt Id: P29994)
Reactivity Reacts with: human (Q14643), rat (P29994), mouse (P11881), cow.
Other species not tested yet.
Matching control protein/peptide 117-0P
Remarks

WB: Due to its large size, IP3 receptor requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Data sheet 117_003.pdf

References for IP3-receptor type1 - 117 003

Disruption of endoplasmic reticulum-mitochondria tethering proteins in post-mortem Alzheimer's disease brain.
Lau DHW, Paillusson S, Hartopp N, Rupawala H, Mórotz GM, Gomez-Suaga P, Greig J, Troakes C, Noble W, Miller CCJ
Neurobiology of disease (2020) : 105020. 117 003 WB; tested species: human
Stimulating VAPB-PTPIP51 ER-mitochondria tethering corrects FTD/ALS mutant TDP43 linked Ca2+ and synaptic defects.
Markovinovic A, Martín-Guerrero SM, Mórotz GM, Salam S, Gomez-Suaga P, Paillusson S, Greig J, Lee Y, Mitchell JC, Noble W, Miller CCJ, et al.
Acta neuropathologica communications (2024) 121: 32. 117 003 ICC; tested species: human
Cat. No.: 117 003
Amount: 50 µg
Price: $455.00
Disruption of endoplasmic reticulum-mitochondria tethering proteins in post-mortem Alzheimer's disease brain.
Lau DHW, Paillusson S, Hartopp N, Rupawala H, Mórotz GM, Gomez-Suaga P, Greig J, Troakes C, Noble W, Miller CCJ
Neurobiology of disease (2020) : 105020. 117 003 WB; tested species: human
Stimulating VAPB-PTPIP51 ER-mitochondria tethering corrects FTD/ALS mutant TDP43 linked Ca2+ and synaptic defects.
Markovinovic A, Martín-Guerrero SM, Mórotz GM, Salam S, Gomez-Suaga P, Paillusson S, Greig J, Lee Y, Mitchell JC, Noble W, Miller CCJ, et al.
Acta neuropathologica communications (2024) 121: 32. 117 003 ICC; tested species: human
WB
IHC
IHC-P
Background
Inositol 1, 4, 5 - trisphophate InsP3 is an intracellular messenger that triggers release of Ca2+ from intracellular stores. InsP3 acts by binding to specific receptors localized to endoplasmic reticulum.
There are at least three types of InsP3 receptors, of which the type 1 receptor is the most abundant. All three receptors appear to be widely expressed. Highest levels of the type 1 InsP3 receptors are found in neurons, with very high expression in the Purkinje cells of the cerebellum.
Other products for this target