Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

IgE antibody - 372 011C2

IgEs are involved in defense against parasites and allergic response
Mouse monoclonal purified IgG
Cat. No.: 372 011C2
Amount: 100 µg
Price: $465.00
Cat. No. 372 011C2 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
FACS: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.

Label Cyanine 2
Clone 164G11
Subtype IgG2a (κ light chain)
Immunogen Purified human basophile granulocytes.
Reactivity Reacts with: human.
Other species not tested yet.
Remarks

FACS: This antibody does not activate basophiles upon binding.

Data sheet 372_011c2.pdf
Cat. No.: 372 011C2
Amount: 100 µg
Price: $465.00
Background
Immunglobulin E or IgE is the most rare immunoglobulin class in mammalian serum. IgEs mediate immunity against parasites like helminths and protozoa. They also play a major role in type I hypersensitivity in various allergic diseases.