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Home > Products > 372 011C2

IgE antibody - 372 011C2

IgEs are involved in defense against parasites and allergic response
Mouse monoclonal purified IgG
Cat. No.: 372 011C2
Amount: 100 µg
Price: $465.00
Cat. No. 372 011C2 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 2.

Fluorescence labeled antibodies conjugated to Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

 Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
FACS: yes (see remarks)
Label Cyanine 2
Clone 164G11
Subtype IgG2a (κ light chain)
Immunogen Purified human basophile granulocytes.
Reactivity Reacts with: human.
Other species not tested yet.
Remarks

FACS: This antibody does not activate basophiles upon binding.
Data sheet 372_011c2.pdf
Cat. No.: 372 011C2
Amount: 100 µg
Price: $465.00
ICC
Background
Immunglobulin E or IgE is the most rare immunoglobulin class in mammalian serum. IgEs mediate immunity against parasites like helminths and protozoa. They also play a major role in type I hypersensitivity in various allergic diseases.
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