Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 372 011BT

IgE antibody - 372 011BT

IgEs are involved in defense against parasites and allergic response
Mouse monoclonal purified IgG
Cat. No.: 372 011BT
Amount: 100 µg
Price: $465.00
Cat. No. 372 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks)
IP: not tested yet
ICC: 1 : 500
IHC: not tested yet
IHC-P: not recommended
ELISA: yes ; suitable as capture antibody
FACS: yes (see remarks)
Label biotin
Clone 164G11
Subtype IgG2a (κ light chain)
Immunogen Purified human basophile granulocytes.
Reactivity Reacts with: human.
Other species not tested yet.
Remarks

WB: Unreduced and non-boiled samples are recommended.
FACS: This antibody does not activate basophiles upon binding.
Data sheet 372_011bt.pdf
Cat. No.: 372 011BT
Amount: 100 µg
Price: $465.00
Background
Immunglobulin E or IgE is the most rare immunoglobulin class in mammalian serum. IgEs mediate immunity against parasites like helminths and protozoa. They also play a major role in type I hypersensitivity in various allergic diseases.
Other products for this target