Synaptic Systems

Cat. No.: HS-378 011

Amount: 100 µg

Price: $415.00

IHC-P

Background

STAT 6 (Signal Transducer and Activator of Transcription 6) is a member of the STAT family of transcription factors. Gene fusion of NGFI-A binding protein 2 (NAB 2) with STAT 6, which converts the transcriptional repressor NAB 2 into a transcriptional activator, was detected as driver mutation in solitary fibrous tumors (SFT). The NAB 2-STAT 6 fusion shows a consistent nuclear reallocation in STAT 6 immunohistochemistry. STAT 6 is a highly sensitive and specific immunohistochemical marker for most SFT and can be helpful to distinguish this tumor type from histologic mimics.
Although considered to be a distinct entity from meningeal SFT in the WHO Classification of CNS Tumours (2007), nuclear STAT 6 expression is additionally found in meningeal hemangiopericytoma (HPC). STAT 6 is a reliable marker to distinguish meningeal solitary fibrous tumors-hemangiopericytomas versus meningiomas.
This antibody is part of the HistoSure^® product line, specifically developed and tested for human pathology.

1 ) Macagno N et al., Am. J. Surg. Pathol. (2016)
2 ) Fritchie KJ et al., J. Neuropathol. Exp. Neurol. (2016)
3 ) Guner G et al., Hum. Pathol. (2016)
4 ) Doyle LA et al., Mod. Pathol. (2014)
5 ) Schweizer L et al., Acta Neuropathol. (2013)
6 ) Robinson DR et al., Nat. Genet. (2013)

Cat. No. HS-378 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 200 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	37C12
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 832 to 847 from human Stat6 (UniProt Id: P42226)
Reactivity	Reacts with: human (P42226). Other species not tested yet.
Data sheet	hs-378_011.pdf

STAT6 antibody - HS-378 011