Ig lambda light chain antibody - HS-349 117

Ig lambda light chain is a useful marker for the classification of B-cell lymphoma

Rat monoclonal purified IgG

Cat. No.: HS-349 117

Amount: 100 µg

Price: $415.00

Cat. No. HS-349 117	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 1000 up to 1 : 10000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	23A1
Subtype	IgG2a
Immunogen	Recombinant protein corresponding to AA 1 to 216 from human IgG λ light chain
Reactivity	Reacts with: human. Other species not tested yet.
Specificity	Specific for λ-light chain of human immunoglobulins. No cross-reactivity to κ light chains.
Data sheet	hs-349_117.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-349 117

Amount: 100 µg

Price: $415.00

IHC-P

Background

Immunoglobulin (Ig) molecules consist of heavy chains and light chains of either lambda (λ) or kappa (κ) type, with κ light chains being the more common form. Immunoglobulines of the IgG, IgD and IgE type are composed of two heavy and two light chains. IgA occurs as dimeric and IgM as pentameric Ig complexes.

1 ) Westermark P et al., Ups. J. Med. Sci. (2012)

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