Synaptic Systems

Cat. No.: HS-398 002

Amount: 200 µl

Price: $350.00

IHC-P

Background

Expression of the nuclear protein Ki 67 is strictly associated with cell proliferation and preferentially expressed during the late G1, S, G2 and M phases of the cell cycle. Resting cells (G0 phase) lack Ki 67 expression .
Immunohistochemical detection of Ki 67 is a simple and reproducible method to determine the tumour proliferative index and is a predictive and prognostic biomarker in certain types of human cancer, such as breast cancer, gastric cancer or prostate cancer. Moreover, higher Ki 67 scores may be associated with increased tumor sensitivity to radiation therapy and chemotherapy.
In preclinical and clinical studies Ki 67 expression is used as a pharmacodynamic biomarker. Absence of a decrease in Ki 67 early in treatment might be predictive of therapeutic failure.

1 ) Nakashoji A et al., Oncol Lett (2017)
2 ) Berlin A et al., Urol. Oncol. (2017)
3 ) Badary DM et al., Pathophysiology (2017)
4 ) Ishibashi N et al., Radiat Oncol (2017)
5 ) El Amine O et al., Tunis Med (2016)
6 ) Inwald EC et al., Breast Cancer Res. Treat. (2013)
7 ) Dowsett M et al., J. Natl. Cancer Inst. (2011)
8 ) Nishimura R et al., Breast Cancer (2010)
9 ) Scholzen T et al., J. Cell. Physiol. (2000)
10 ) Hall PA et al., J. Pathol. (1988)

Other products for this target

Cat. No. HS-398 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 2000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1251 to 1271 from human Ki67 (UniProt Id: P46013)
Reactivity	Reacts with: human (P46013). No signal: mouse (E9PVX6), rat (D4A0Y6). Other species not tested yet.
Data sheet	hs-398_002.pdf

Ki67 antibody human specific - HS-398 002