Ig kappa light chain antibody - HS-346 011BT

Ig kappa light chain is a useful marker for the classification of B-cell lymphoma

Mouse monoclonal purified IgG

Cat. No.: HS-346 011BT

Amount: 100 µg

Price: $465.00

Cat. No. HS-346 011BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: gallery IHC-P: 1 : 1000 up to 1 : 10000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	190F11
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 130 to 235 from human IgG κ LC
Reactivity	Reacts with: human. Other species not tested yet.
Specificity	Detects the κ light chain of human immunoglobulins. No cross-reactivity to λ light chains.
Data sheet	hs-346_011bt.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-346 011BT

Amount: 100 µg

Price: $465.00

IHC-P

Background

Immunoglobulin (Ig) molecules consist of heavy chains and light chains of either lambda (λ) or kappa (κ) type, with κ light chains being the more common form. Immunoglobulines of the IgG, IgD and IgE type are composed of two heavy and two light chains. IgA occurs as dimeric and IgM as pentameric Ig complexes.

1 ) Westermark P et al., Ups. J. Med. Sci. (2012)

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