CD4 antibody mouse specific - HS-360 017

CD4 is a T-cell surface glycoprotein

Rat monoclonal purified IgG

Cat. No.: HS-360 017

Amount: 200 µl

Price: $415.00

Cat. No. HS-360 017	200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 100 (see remarks) gallery IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	GHH4/114B4
Subtype	IgG2b
Immunogen	Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)
Reactivity	Reacts with: mouse (P06332). No signal: human (P01730), rat (P05540). Other species not tested yet.
Remarks	IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet	hs-360_017.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

References for CD4 - HS-360 017

Genomic control of inflammation in experimental atopic dermatitis.
Liu Y, Zienkiewicz J, Qiao H, Gibson-Corley KN, Boyd KL, Veach RA, Hawiger J
Scientific reports (2022) 121: 18891. HS-360 017 IHC-P; tested species: mouse

Cat. No.: HS-360 017

Amount: 200 µl

Price: $415.00

IHC

IHC-P

Background

CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD4 is a co-receptor that assists the T-cell receptor (TCR) in communicating with an antigen-presenting cell.

1 ) Ansari-Lari MA et al., Genome Res. (1996)
2 ) Brady RL et al., Science (1993)
3 ) Prince HE et al., Diagn Clin Immunol (1987)
4 ) Fleischer B et al., J. Immunol. (1986)

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