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Home > Products > 352 003

Hacd3 antibody - 352 003

Hacd3 is involved in VLCFA synthesis
Rabbit polyclonal purified antibody
Cat. No.: 352 003
Amount: 50 µg
Price: $375.00
Cat. No. 352 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 149 from rat Hacd3 (UniProt Id: D4ABI7)
Reactivity Reacts with: rat (D4ABI7), mouse (Q8K2C9).
Other species not tested yet.
Data sheet 352_003.pdf
Cat. No.: 352 003
Amount: 50 µg
Price: $375.00
WB
Background
Very long-chain fatty acids are produced through a four-step cycle. Hacd 3 the very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3 catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of two carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. The enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.