HA-tag antibody - 245 003C3

The HA-Tag is a widely used epitope tag

Rabbit polyclonal purified antibody

Cat. No.: 245 003C3

Amount: 50 µg

Price: $480.00

Cat. No. 245 003C3	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster 550. Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established fluorescence detection systems. Oyster 550: λ_ex 551 nm / λ_em 570 nm Oyster 650: λ_ex 651 nm / λ_em 671 nm Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates. Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex^®(Merck Chemicals) is not compatible with Oyster dyes!
Applications	WB: N/A IP: N/A ICC: 1 : 200 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Oyster 550
Immunogen	Synthetic peptide corresponding to AA 98 to 108 from human HA-tag
Specificity	Specific for HA-tag, hemaglutinin.
Matching control protein/peptide	245-0P
Data sheet	245_003c3.pdf

References for HA-tag - 245 003C3

TGF-β-Induced Phosphorylation of Usp9X Stabilizes Ankyrin-G and Regulates Dendritic Spine Development and Maintenance.
Yoon S, Parnell E, Penzes P
Cell reports (2020) 318: 107685. 245 003C3 ICC; tested species: mouse

Usp9X Controls Ankyrin-Repeat Domain Protein Homeostasis during Dendritic Spine Development.
Yoon S, Parnell E, Kasherman M, Forrest MP, Myczek K, Premarathne S, Sanchez Vega MC, Piper M, Burne THJ, Jolly LA, Wood SA, et al.
Neuron (2020) 1053: 506-521.e7. 245 003C3 ICC; tested species: mouse

Cat. No.: 245 003C3

Amount: 50 µg

Price: $480.00

ICC

Background

The surface glycoprotein hemaglutinin (HA) of the human influenza virus is essential for the infectivity of the virus. The HA-tag corresponds to amino acids 98-106 of this protein and has been widely used as an epitope tag in protein expression vectors. It can be employed for the detection and immunoisolation of proteins using immunoblotting, immunoprecipitation and immunostaining methods and has been shown to have only neglectable influence on the biological properties of the tagged protein.

1 ) Xie LY et al., Endocrinology (1998)
2 ) Lauber J et al., EMBO J. (1996)
3 ) Howard M et al., Am. J. Physiol. (1995)
4 ) Handley-Gearhart PM et al., J. Biol. Chem. (1994)
5 ) Chen YT et al., Proc. Natl. Acad. Sci. U.S.A. (1993)

Protocols

ICC

OTHER

Preabsorbtion

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