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Home > Products > 245 003BT

HA-tag antibody - 245 003BT

The HA-Tag is a widely used epitope tag
Rabbit polyclonal purified antibody
Cat. No.: 245 003BT
Amount: 50 µg
Price: $480.00
Cat. No. 245 003BT 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
Label biotin
Immunogen Synthetic peptide corresponding to AA 98 to 108 from human HA-tag
Specificity Specific for HA-tag, hemaglutinin.
Matching control protein/peptide 245-0P
Data sheet 245_003bt.pdf
Cat. No.: 245 003BT
Amount: 50 µg
Price: $480.00
WB
ICC
Background
The surface glycoprotein hemaglutinin (HA) of the human influenza virus is essential for the infectivity of the virus. The HA-tag corresponds to amino acids 98-106 of this protein and has been widely used as an epitope tag in protein expression vectors. It can be employed for the detection and immunoisolation of proteins using immunoblotting, immunoprecipitation and immunostaining methods and has been shown to have only neglectable influence on the biological properties of the tagged protein.
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