HA-tag antibody - 245 003BT

The HA-Tag is a widely used epitope tag

Rabbit polyclonal purified antibody

Cat. No.: 245 003BT

Amount: 50 µg

Price: $480.00

Cat. No. 245 003BT	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 200 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Immunogen	Synthetic peptide corresponding to AA 98 to 108 from human HA-tag
Specificity	Specific for HA-tag, hemaglutinin.
Matching control protein/peptide	245-0P
Data sheet	245_003bt.pdf

Cat. No.: 245 003BT

Amount: 50 µg

Price: $480.00

ICC

Background

The surface glycoprotein hemaglutinin (HA) of the human influenza virus is essential for the infectivity of the virus. The HA-tag corresponds to amino acids 98-106 of this protein and has been widely used as an epitope tag in protein expression vectors. It can be employed for the detection and immunoisolation of proteins using immunoblotting, immunoprecipitation and immunostaining methods and has been shown to have only neglectable influence on the biological properties of the tagged protein.

1 ) Xie LY et al., Endocrinology (1998)
2 ) Lauber J et al., EMBO J. (1996)
3 ) Howard M et al., Am. J. Physiol. (1995)
4 ) Handley-Gearhart PM et al., J. Biol. Chem. (1994)
5 ) Chen YT et al., Proc. Natl. Acad. Sci. U.S.A. (1993)

Protocols

ICC

OTHER

Preabsorbtion

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