Synaptic Systems - Glycine transporter1

Cat. No.: 272 103

Amount: 50 µg

Price: $385.00

Cat. No. 272 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 1000 up to 1 : 2000 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 649 to 692 from mouse Glycine transporter1 (UniProt Id: P28571)
Reactivity	Reacts with: rat (P28572), mouse (P28571). Other species not tested yet.
Remarks	WB: Glycine transporter 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	272_103.pdf

References for Glycine transporter1 - 272 103

GlyT1 Encephalopathy: Characterization of presumably disease causing GlyT1 mutations.
Hauf K, Barsch L, Bauer D, Buchert R, Armbruster A, Frauenfeld L, Grasshoff U, Eulenburg V
Neurochemistry international (2020) : 104813. 272 103 WB, ICC; tested species: human

Vigabatrin-Induced Retinal Functional Alterations and Second-Order Neuron Plasticity in C57BL/6J Mice.
Chan K, Hoon M, Pattnaik BR, Ver Hoeve JN, Wahlgren B, Gloe S, Williams J, Wetherbee B, Kiland JA, Vogel KR, Jansen E, et al.
Investigative ophthalmology & visual science (2020) 612: 17. 272 103 IHC; tested species: mouse

Opposing effects of an atypical glycinergic and substance P transmission on interpeduncular nucleus plasticity.
Melani R, Von Itter R, Jing D, Koppensteiner P, Ninan I
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2019) : . 272 103 IHC; tested species: mouse

Cat. No.: 272 103

Amount: 50 µg

Price: $385.00

IHC

IHC-P

Background

Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem. Two differentially expressed glycine transporters, GLYT 1 and GLYT 2, regulate the extracellular concentration of this neuroactive amino acid in the synaptic cleft.
GLYT 1 is expressed in both neurons as well as in glia with high expression levels in the main olfactory bulb, cerebellum, brainstem and spinal cord and low expression in other brain regions. It has been hypothesized to regulate glycine levels in NMDA receptor-mediated neurotransmission.
GLYT 2 shows an axonal localization and is mainly expressed in spinal cord, brain-stem and cerebellum.

1 ) Kurolap A et al., Am. J. Hum. Genet. (2016)
2 ) James VM et al., Biol. Chem. (2012)
3 ) Tsai G et al., Proc. Natl. Acad. Sci. U.S.A. (2004)
4 ) Gomeza J et al., Neuron (2003)
5 ) Geerlings A et al., J. Biol. Chem. (2001)
6 ) Martínez-Maza R et al., J. Biol. Chem. (2001)
7 ) Zafra F et al., J. Neurosci. (1995)
8 ) Adams RH et al., J. Neurosci. (1995)
9 ) Jursky F et al., J. Neurochem. (1995)

Protocols

IHC

IHC-P

Glycine transporter1 antibody - 272 103

References for Glycine transporter1 - 272 103