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Glycine receptor antibody - 146 008

An inhibitory receptor in the nervous system
Rabbit monoclonal recombinant IgG
Cat. No.: 146 008
Amount: 50 µg
Price: $415.00
Cat. No. 146 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 up to 1 : 5000 (see remarks) gallery  
IHC-P: 1 : 500 gallery  
Clone RbmAb4a
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity Reacts with: human (P23415, P23416, P48167), rat (P07727, P22771, P20781), mouse (Q64018, Q7TNC8, P48168), pig, zebrafish.
Other species not tested yet.
Specificity Specific for all glycine receptor subunits.
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb4a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. It also carries a Strep-tag at the C-terminus of the heavy chain.The antibody has been expressed in mammalian cells.
IHC: Tissue sections require additional antigen retrieval with methanol/acetic acid prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001).

Data sheet 146_008.pdf

References for Glycine receptor - 146 008

Role of the Glycine Receptor β Subunit in Synaptic Localization and Pathogenicity in Severe Startle Disease.
Wiessler AL, Hasenmüller AS, Fuhl I, Mille C, Cortes Campo O, Reinhard N, Schenk J, Heinze KG, Schaefer N, Specht CG, Villmann C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 442: . 146 008 WB; tested species: mouse
Contribution of GlyR α3 Subunits to the Sensitivity and Effect of Ethanol in the Nucleus Accumbens.
San Martin LS, Armijo-Weingart L, Araya A, Yévenes GE, Harvey RJ, Aguayo LG
Frontiers in molecular neuroscience (2021) 14: 756607. 146 008 WB; tested species: mouse
Presence of ethanol-sensitive and ethanol-insensitive glycine receptors in the ventral tegmental area and prefrontal cortex in mice.
Araya A, Gallegos S, Viveros R, San Martin L, Muñoz B, Harvey RJ, Zeilhofer HU, Aguayo LG
British journal of pharmacology (2021) 17823: 4691-4707. 146 008 WB; tested species: mouse
Ethanol consumption and sedation are altered in mice lacking the glycine receptor α2 subunit.
San Martin L, Gallegos S, Araya A, Romero N, Morelli G, Comhair J, Harvey RJ, Rigo JM, Brone B, Aguayo LG
British journal of pharmacology (2020) 17717: 3941-3956. 146 008 WB; tested species: mouse
A zebrafish gephyrinb mutant distinguishes synaptic and enzymatic functions of Gephyrin.
Brennan EJ, Monk KR, Li J
Neural development (2024) 191: 14. 146 008 IHC; tested species: zebrafish
Synaptic input and Ca2+ activity in zebrafish oligodendrocyte precursor cells contribute to myelin sheath formation.
Li J, Miramontes TG, Czopka T, Monk KR
Nature neuroscience (2024) 272: 219-231. 146 008 IHC; tested species: zebrafish
Brain volume increase and neuronal plasticity underly predator-induced morphological defense expression in Daphnia longicephala.
Graeve A, Ioannidou I, Reinhard J, Görl DM, Faissner A, Weiss LC
Scientific reports (2021) 111: 12612. 146 008 IHC
Cat. No.: 146 008
Amount: 50 µg
Price: $415.00
Role of the Glycine Receptor β Subunit in Synaptic Localization and Pathogenicity in Severe Startle Disease.
Wiessler AL, Hasenmüller AS, Fuhl I, Mille C, Cortes Campo O, Reinhard N, Schenk J, Heinze KG, Schaefer N, Specht CG, Villmann C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 442: . 146 008 WB; tested species: mouse
Contribution of GlyR α3 Subunits to the Sensitivity and Effect of Ethanol in the Nucleus Accumbens.
San Martin LS, Armijo-Weingart L, Araya A, Yévenes GE, Harvey RJ, Aguayo LG
Frontiers in molecular neuroscience (2021) 14: 756607. 146 008 WB; tested species: mouse
Presence of ethanol-sensitive and ethanol-insensitive glycine receptors in the ventral tegmental area and prefrontal cortex in mice.
Araya A, Gallegos S, Viveros R, San Martin L, Muñoz B, Harvey RJ, Zeilhofer HU, Aguayo LG
British journal of pharmacology (2021) 17823: 4691-4707. 146 008 WB; tested species: mouse
Ethanol consumption and sedation are altered in mice lacking the glycine receptor α2 subunit.
San Martin L, Gallegos S, Araya A, Romero N, Morelli G, Comhair J, Harvey RJ, Rigo JM, Brone B, Aguayo LG
British journal of pharmacology (2020) 17717: 3941-3956. 146 008 WB; tested species: mouse
A zebrafish gephyrinb mutant distinguishes synaptic and enzymatic functions of Gephyrin.
Brennan EJ, Monk KR, Li J
Neural development (2024) 191: 14. 146 008 IHC; tested species: zebrafish
Synaptic input and Ca2+ activity in zebrafish oligodendrocyte precursor cells contribute to myelin sheath formation.
Li J, Miramontes TG, Czopka T, Monk KR
Nature neuroscience (2024) 272: 219-231. 146 008 IHC; tested species: zebrafish
Brain volume increase and neuronal plasticity underly predator-induced morphological defense expression in Daphnia longicephala.
Graeve A, Ioannidou I, Reinhard J, Görl DM, Faissner A, Weiss LC
Scientific reports (2021) 111: 12612. 146 008 IHC
Background
The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.