Glycogen phosphorylase antibody - 255 003

Glycogen phosphorylase is a key player in glycogen degradation

Rabbit polyclonal purified antibody

Cat. No.: 255 003

Amount: 50 µg

Price: $455.00

Cat. No. 255 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 826 to 842 from mouse Glycogen phosphorylase (UniProt Id: Q8CI94)
Reactivity	Reacts with: rat (P53534), mouse (Q8CI94). Other species not tested yet.
Matching control protein/peptide	255-0P
Data sheet	255_003.pdf

Cat. No.: 255 003

Amount: 50 µg

Price: $455.00

ICC

IHC

Background

The homodimeric protein glycogen phosphorylase is the key enzyme in glycogen degradation. Three isoforms dominating either muscle, liver or brain tissue have been described. PYGB is the brain specific isoform that is mainly expressed in astrocytes. These glia cells contain large amounts of glycogen that serve as an energy reserve.

1 ) Pfeiffer-Guglielmi B et al., Glia (2005)
2 ) Pfeiffer-Guglielmi B et al., J. Neurochem. (2003)
3 ) Hudson JW et al., Biochim. Biophys. Acta (1993)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

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