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Home > Products > 367 005

Glutamine synthetase antibody - 367 005

GS is an enzyme for glutamine synthesis and ammonia detoxification, a marker for astrocytes
Guinea pig polyclonal purified antibody
Cat. No.: 367 005
Amount: 50 µg
Price: $465.00
Cat. No. 367 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Full-length recombinant mouse Glutamine synthetase (UniProt Id: P15105)
Reactivity Reacts with: rat (P09606), mouse (P15105).
Other species not tested yet.
Data sheet 367_005.pdf

References for Glutamine synthetase - 367 005

Caloric restriction triggers morphofunctional remodeling of astrocytes and enhances synaptic plasticity in the mouse hippocampus.
Popov A, Denisov P, Bychkov M, Brazhe A, Lyukmanova E, Shenkarev Z, Lazareva N, Verkhratsky A, Semyanov A
Cell death & disease (2020) 113: 208. 367 005 WB; tested species: mouse
The Musashi-1-type 2 deiodinase pathway regulates astrocyte proliferation.
Mohácsik P, Halmos E, Dorogházi B, Ruska Y, Wittmann G, Bianco AC, Fekete C, Gereben B
The Journal of biological chemistry (2024) 3007: 107477. 367 005 IHC; tested species: mouse
The normalizing effects of the CYP46A1 activator efavirenz on retinal sterol levels and risk factors for glaucoma in Apoj-/- mice.
El-Darzi N, Mast N, Li Y, Dailey B, Kang M, Rhee DJ, Pikuleva IA
Cellular and molecular life sciences : CMLS (2023) 807: 194. 367 005 IHC; tested species: mouse
Microglia alter the threshold of spreading depolarization and related potassium uptake in the mouse brain.
Varga DP, Menyhárt Á, Pósfai B, Császár E, Lénárt N, Cserép C, Orsolits B, Martinecz B, Szlepák T, Bari F, Farkas E, et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2020) : 271678X19900097. 367 005 IHC; tested species: mouse
Changes in the transcriptional fingerprint of satellite glial cells following peripheral nerve injury.
Jager SE, Pallesen LT, Richner M, Harley P, Hore Z, McMahon S, Denk F, Vaegter CB
Glia (2020) : . 367 005 IHC; tested species: mouse
Lysophosphatidic acid activates satellite glia cells and Schwann cells.
Robering JW, Gebhardt L, Wolf K, Kühn H, Kremer AE, Fischer MJM
Glia (2019) : . 367 005 IHC; tested species: mouse
Occurrence of Transmembrane Protein 119 in the Retina is Not Restricted to the Microglia: An Immunohistochemical Study.
Su N, März S, Plagemann T, Cao J, Schnittler HJ, Eter N, Heiduschka P
Translational vision science & technology (2019) 86: 29. 367 005 IHC; tested species: mouse
Cat. No.: 367 005
Amount: 50 µg
Price: $465.00
Caloric restriction triggers morphofunctional remodeling of astrocytes and enhances synaptic plasticity in the mouse hippocampus.
Popov A, Denisov P, Bychkov M, Brazhe A, Lyukmanova E, Shenkarev Z, Lazareva N, Verkhratsky A, Semyanov A
Cell death & disease (2020) 113: 208. 367 005 WB; tested species: mouse
The Musashi-1-type 2 deiodinase pathway regulates astrocyte proliferation.
Mohácsik P, Halmos E, Dorogházi B, Ruska Y, Wittmann G, Bianco AC, Fekete C, Gereben B
The Journal of biological chemistry (2024) 3007: 107477. 367 005 IHC; tested species: mouse
The normalizing effects of the CYP46A1 activator efavirenz on retinal sterol levels and risk factors for glaucoma in Apoj-/- mice.
El-Darzi N, Mast N, Li Y, Dailey B, Kang M, Rhee DJ, Pikuleva IA
Cellular and molecular life sciences : CMLS (2023) 807: 194. 367 005 IHC; tested species: mouse
Microglia alter the threshold of spreading depolarization and related potassium uptake in the mouse brain.
Varga DP, Menyhárt Á, Pósfai B, Császár E, Lénárt N, Cserép C, Orsolits B, Martinecz B, Szlepák T, Bari F, Farkas E, et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2020) : 271678X19900097. 367 005 IHC; tested species: mouse
Changes in the transcriptional fingerprint of satellite glial cells following peripheral nerve injury.
Jager SE, Pallesen LT, Richner M, Harley P, Hore Z, McMahon S, Denk F, Vaegter CB
Glia (2020) : . 367 005 IHC; tested species: mouse
Lysophosphatidic acid activates satellite glia cells and Schwann cells.
Robering JW, Gebhardt L, Wolf K, Kühn H, Kremer AE, Fischer MJM
Glia (2019) : . 367 005 IHC; tested species: mouse
Occurrence of Transmembrane Protein 119 in the Retina is Not Restricted to the Microglia: An Immunohistochemical Study.
Su N, März S, Plagemann T, Cao J, Schnittler HJ, Eter N, Heiduschka P
Translational vision science & technology (2019) 86: 29. 367 005 IHC; tested species: mouse
WB
IHC
IHC-P
Background
Glutamine synthetase, also referred to as Glutamate-ammonia ligase or GS, is an enzyme that catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. It is present predominantly in brain, kidneys, and liver. In the brain, it is particularly found in astrocytes.
Glutamine synthetase plays a pivotal role in glutamate and glutamine homoeostasis, and it is largely responsible for the removal of both blood-derived and metabolically generated ammonia, preventing neurotoxicity. It is also a key enzyme in the recycling of the neurotransmitter glutamate.
Several studies indicated that the expression, distribution, and activity of brain glutamine synthetase is altered in some brain disorders, including Alzheimer's disease, schizophrenia, depression, suicidality, and mesial temporal lobe epilepsy (MTLE).
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