GLUT4 peptide - 235-0P

GLUT4 is a glucose transporter involved in insulin stimulated glucose uptake

control peptide

Cat. No.: 235-0P

Amount: 100 µg

Price: $105.00

Cat. No. 235-0P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 495 to 509 from human GLUT4 (UniProt Id: P14672)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	235 003
Remarks	This control peptide consists of the synthetic peptide (aa 495-509 of human GLUT 4) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	235-0p.pdf

Cat. No.: 235-0P

Amount: 100 µg

Price: $105.00

Background

Insulin stimulates glucose transport into muscle and fat cells by the redistribution of the glucose transporters 1 and 4 (GLUT 1 and GLUT 4) from intracellular membrane compartments to the cell surface via GLUT carrying vesicles.
Formation of soluble SNARE complexes mediate the docking and fusion of GLUT 4-containing vesicles with the plasma membrane.

1 ) Fukuda N et al., Diabetes (2009)
2 ) Li Y et al., Biochem. Biophys. Res. Commun. (2007)
3 ) Foster LJ et al., Am. J. Physiol., Cell Physiol. (2000)
4 ) Thurmond DC et al., J. Biol. Chem. (1998)
5 ) Haney PM et al., J. Cell Biol. (1991)

Protocols

OTHER

Preadsorbtion

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