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Home > Products > 263 003

Giantin antibody - 263 003

Giantin is specifically expressed in the Golgi complex
Rabbit polyclonal purified antibody
Cat. No.: 263 003
Amount: 50 µg
Price: $375.00
Cat. No. 263 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 500 gallery  
DNA-PAINT: yes (see remarks)
Immunogen Recombinant protein corresponding to AA 1 to 427 from rat Giantin (UniProt Id: G3V6A8)
Reactivity Reacts with: rat (G3V6A8), mouse.
No signal: zebrafish.
Other species not tested yet.
Remarks

WB: Due to its large size, Giantin requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet 263_003.pdf

References for Giantin - 263 003

VAPB depletion alters neuritogenesis and phosphoinositide balance in motoneuron-like cells: relevance to VAPB-linked ALS.
Genevini P, Colombo MN, Venditti R, Marcuzzo S, Colombo SF, Bernasconi P, De Matteis MA, Borgese N, Navone F
Journal of cell science (2019) : . 263 003 ICC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 263 003 DNA-PAINT; tested species: rat
Cat. No.: 263 003
Amount: 50 µg
Price: $375.00
VAPB depletion alters neuritogenesis and phosphoinositide balance in motoneuron-like cells: relevance to VAPB-linked ALS.
Genevini P, Colombo MN, Venditti R, Marcuzzo S, Colombo SF, Bernasconi P, De Matteis MA, Borgese N, Navone F
Journal of cell science (2019) : . 263 003 ICC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 263 003 DNA-PAINT; tested species: rat
WB
ICC
IHC
IHC-P
Background
In eukaryotic cells the Golgi complex consists of stacked cisternae. Giantin, also referred to as macrogolgin or p400, is a single pass membrane protein involved in tethering these cisternae by cross-bridge formation. Its large cytoplasmic N-terminus is involved in the docking of COPI vesicles via p115 to the Golgi membrane.
Systemic lupus erythematosus and Sjögren's syndrome correlate with autoimmune antibodies against Golgi-compartment proteins with giantin being the most common autoantigen.
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