Giantin antibody - 263 003

Giantin is specifically expressed in the Golgi complex

Rabbit polyclonal purified antibody

Cat. No.: 263 003

Amount: 50 µg

Price: $375.00

Cat. No. 263 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 500 gallery DNA-PAINT: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Immunogen	Recombinant protein corresponding to AA 1 to 427 from rat Giantin (UniProt Id: G3V6A8)
Reactivity	Reacts with: rat (G3V6A8), mouse. No signal: zebrafish. Other species not tested yet.
Remarks	WB: Due to its large size, Giantin requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen. DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet	263_003.pdf

References for Giantin - 263 003

VAPB depletion alters neuritogenesis and phosphoinositide balance in motoneuron-like cells: relevance to VAPB-linked ALS.
Genevini P, Colombo MN, Venditti R, Marcuzzo S, Colombo SF, Bernasconi P, De Matteis MA, Borgese N, Navone F
Journal of cell science (2019) : . 263 003 ICC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 263 003 DNA-PAINT; tested species: rat

Cat. No.: 263 003

Amount: 50 µg

Price: $375.00

ICC

IHC

IHC-P

Background

In eukaryotic cells the Golgi complex consists of stacked cisternae. Giantin, also referred to as macrogolgin or p400, is a single pass membrane protein involved in tethering these cisternae by cross-bridge formation. Its large cytoplasmic N-terminus is involved in the docking of COPI vesicles via p115 to the Golgi membrane.
Systemic lupus erythematosus and Sjögren's syndrome correlate with autoimmune antibodies against Golgi-compartment proteins with giantin being the most common autoantigen.

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2 ) Nozawa K et al., Arthritis Res. Ther. (2004)
3 ) Alvarez C et al., J. Biol. Chem. (2001)
4 ) Sönnichsen B et al., J. Cell Biol. (1998)
5 ) Seelig HP et al., Mol. Cell. Biol. (1994)
6 ) Linstedt AD et al., Mol. Biol. Cell (1993)

Protocols

ICC

IHC

IHC-P

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