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Home > Products > 132 111

GFP antibody - 132 111

GFP is a widely used green fluorescent protein tag
Mouse monoclonal purified IgG
Cat. No.: 132 111
Amount: 100 µg
Price: $415.00
Cat. No. 132 111 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: not recommended (see remarks)
ICC: yes (methanol fixated material only) (see remarks)
IHC: not recommended
IHC-P: not tested yet
Clone 101G4
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Epitop AA 183 to 191 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Remarks

IP: Cat. no. 132 011 or 132 002 is recommended for IP.
ICC: Cat. no. 132 011 or 132 002 is recommended for ICC.
Data sheet 132_111.pdf

References for GFP - 132 111

FOXR1 regulates stress response pathways and is necessary for proper brain development.
Mota A, Waxman HK, Hong R, Lagani GD, Niu SY, Bertherat FL, Wolfe L, Malicdan CM, Markello TC, Adams DR, Gahl WA, et al.
PLoS genetics (2021) 1711: e1009854. 132 111 WB; tested species: human
Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin.
Koestler SA, Steffen A, Nemethova M, Winterhoff M, Luo N, Holleboom JM, Krupp J, Jacob S, Vinzenz M, Schur F, Schlüter K, et al.
Molecular biology of the cell (2013) 2418: 2861-75. 132 111 WB
Microtubules as platforms for assaying actin polymerization in vivo.
Oelkers JM, Vinzenz M, Nemethova M, Jacob S, Lai FP, Block J, Szczodrak M, Kerkhoff E, Backert S, Schlüter K, Stradal TE, et al.
PloS one (2011) 65: e19931. 132 111 WB
Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte.
Kaddai V, Gonzalez T, Keslair F, Grémeaux T, Bonnafous S, Gugenheim J, Tran A, Gual P, Le Marchand-Brustel Y, Cormont M
PloS one (2009) 44: e5257. 132 111 WB
Cat. No.: 132 111
Amount: 100 µg
Price: $415.00
FOXR1 regulates stress response pathways and is necessary for proper brain development.
Mota A, Waxman HK, Hong R, Lagani GD, Niu SY, Bertherat FL, Wolfe L, Malicdan CM, Markello TC, Adams DR, Gahl WA, et al.
PLoS genetics (2021) 1711: e1009854. 132 111 WB; tested species: human
Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin.
Koestler SA, Steffen A, Nemethova M, Winterhoff M, Luo N, Holleboom JM, Krupp J, Jacob S, Vinzenz M, Schur F, Schlüter K, et al.
Molecular biology of the cell (2013) 2418: 2861-75. 132 111 WB
Microtubules as platforms for assaying actin polymerization in vivo.
Oelkers JM, Vinzenz M, Nemethova M, Jacob S, Lai FP, Block J, Szczodrak M, Kerkhoff E, Backert S, Schlüter K, Stradal TE, et al.
PloS one (2011) 65: e19931. 132 111 WB
Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte.
Kaddai V, Gonzalez T, Keslair F, Grémeaux T, Bonnafous S, Gugenheim J, Tran A, Gual P, Le Marchand-Brustel Y, Cormont M
PloS one (2009) 44: e5257. 132 111 WB
WB
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.
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