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GFP antibody - 132 005

GFP is a widely used green fluorescent protein tag
Guinea pig polyclonal purified antibody
Cat. No.: 132 005
Amount: 50 µg
Price: $465.00
Cat. No. 132 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Data sheet 132_005.pdf

References for GFP - 132 005

Reduced dynamin-1 levels in neurons lacking MUNC18-1.
Lammertse HCA, Moro A, Saarloos I, Toonen RF, Verhage M
Journal of cell science (2022) 13522: . 132 005 ICC; tested species: mouse
JNK signalling regulates antioxidant responses in neurons.
Ugbode C, Garnham N, Fort-Aznar L, Evans GJO, Chawla S, Sweeney ST
Redox biology (2020) 37: 101712. 132 005 ICC; tested species: rat
Neuroprotective activity of ursodeoxycholic acid in CHMP2BIntron5 models of frontotemporal dementia.
West RJH, Ugbode C, Fort-Aznar L, Sweeney ST
Neurobiology of disease (2020) 144: 105047. 132 005 ICC; tested species: rat
TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 132 005 ICC, IHC; tested species: rat
Epitope specificity of anti-synapsin autoantibodies: Differential targeting of synapsin I domains.
Mertens R, Melchert S, Gitler D, Schou MB, Saether SG, Vaaler A, Piepgras J, Kochova E, Benfenati F, Ahnert-Hilger G, Ruprecht K, et al.
PloS one (2018) 1312: e0208636. 132 005 ICC; tested species: human
A pH-sensitive closed-loop nanomachine to control hyperexcitability at the single neuron level.
Merolla A, Michetti C, Moschetta M, Vacca F, Ciano L, Emionite L, Astigiano S, Romei A, Horenkamp S, Berglund K, Gross RE, et al.
Nature communications (2024) 151: 5609. 132 005 IHC; tested species: mouse
A Selective Projection from the Subthalamic Nucleus to Parvalbumin-Expressing Interneurons of the Striatum.
Kondabolu K, Doig NM, Ayeko O, Khan B, Torres A, Calvigioni D, Meletis K, Koós T, Magill PJ
eNeuro (2023) 107: . 132 005 IHC; tested species: mouse
Pharmacological signature and target-specificity of inhibitory circuits formed by Martinotti cells in the mouse barrel cortex.
Donato C, Victorino DB, Cabezas C, Aguirre A, Lourenço J, Potier MC, de San Martin JZ, Bacci A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 132 005 IHC; tested species: mouse
TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 132 005 ICC, IHC; tested species: rat
The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells.
Fadiga J, Nystul TG
eLife (2019) 8: . 132 005 IHC; tested species: drosophila
Neuronal activity regulates neurotransmitter switching in the adult brain following light-induced stress.
Meng D, Li HQ, Deisseroth K, Leutgeb S, Spitzer NC
Proceedings of the National Academy of Sciences of the United States of America (2018) : . 132 005 IHC; tested species: rat
Phosphorylated Groucho delays differentiation in the follicle stem cell lineage by providing a molecular memory of EGFR signaling in the niche.
Johnston MJ, Bar-Cohen S, Paroush Z, Nystul TG
Development (Cambridge, England) (2016) 14324: 4631-4642. 132 005 IHC
Cat. No.: 132 005
Amount: 50 µg
Price: $465.00
Reduced dynamin-1 levels in neurons lacking MUNC18-1.
Lammertse HCA, Moro A, Saarloos I, Toonen RF, Verhage M
Journal of cell science (2022) 13522: . 132 005 ICC; tested species: mouse
JNK signalling regulates antioxidant responses in neurons.
Ugbode C, Garnham N, Fort-Aznar L, Evans GJO, Chawla S, Sweeney ST
Redox biology (2020) 37: 101712. 132 005 ICC; tested species: rat
Neuroprotective activity of ursodeoxycholic acid in CHMP2BIntron5 models of frontotemporal dementia.
West RJH, Ugbode C, Fort-Aznar L, Sweeney ST
Neurobiology of disease (2020) 144: 105047. 132 005 ICC; tested species: rat
TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 132 005 ICC, IHC; tested species: rat
Epitope specificity of anti-synapsin autoantibodies: Differential targeting of synapsin I domains.
Mertens R, Melchert S, Gitler D, Schou MB, Saether SG, Vaaler A, Piepgras J, Kochova E, Benfenati F, Ahnert-Hilger G, Ruprecht K, et al.
PloS one (2018) 1312: e0208636. 132 005 ICC; tested species: human
A pH-sensitive closed-loop nanomachine to control hyperexcitability at the single neuron level.
Merolla A, Michetti C, Moschetta M, Vacca F, Ciano L, Emionite L, Astigiano S, Romei A, Horenkamp S, Berglund K, Gross RE, et al.
Nature communications (2024) 151: 5609. 132 005 IHC; tested species: mouse
A Selective Projection from the Subthalamic Nucleus to Parvalbumin-Expressing Interneurons of the Striatum.
Kondabolu K, Doig NM, Ayeko O, Khan B, Torres A, Calvigioni D, Meletis K, Koós T, Magill PJ
eNeuro (2023) 107: . 132 005 IHC; tested species: mouse
Pharmacological signature and target-specificity of inhibitory circuits formed by Martinotti cells in the mouse barrel cortex.
Donato C, Victorino DB, Cabezas C, Aguirre A, Lourenço J, Potier MC, de San Martin JZ, Bacci A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 132 005 IHC; tested species: mouse
TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons.
Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, Buhler E, Sartorelli J, Represa A, Baldelli P, Benfenati F, et al.
Cell death and differentiation (2019) : . 132 005 ICC, IHC; tested species: rat
The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells.
Fadiga J, Nystul TG
eLife (2019) 8: . 132 005 IHC; tested species: drosophila
Neuronal activity regulates neurotransmitter switching in the adult brain following light-induced stress.
Meng D, Li HQ, Deisseroth K, Leutgeb S, Spitzer NC
Proceedings of the National Academy of Sciences of the United States of America (2018) : . 132 005 IHC; tested species: rat
Phosphorylated Groucho delays differentiation in the follicle stem cell lineage by providing a molecular memory of EGFR signaling in the niche.
Johnston MJ, Bar-Cohen S, Paroush Z, Nystul TG
Development (Cambridge, England) (2016) 14324: 4631-4642. 132 005 IHC
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.