Cat. No.: 132 004
Amount: 100 µl
Price:
$365.00
Cat. No. 132 004 |
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 (see remarks) gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.', $event)" style="cursor: help;">EM: yes |
Immunogen | Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212) |
Specificity | Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants. This antibody still recognizes its antigen after heat mediated antigen retrieval. It does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. |
Remarks |
IHC: The unpurified serum tends to stain nuclear structures in WT tissue, that lacks GFP expression. The affinity purified version 132 005 recommended. |
Data sheet | 132_004.pdf |