Synaptic Systems - GABA transporter1

Cat. No.: 274 102

Amount: 200 µl

Price: $365.00

Cat. No. 274 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 gallery ExM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Synthetic peptide corresponding to AA 585 to 599 from mouse GABA transporter1 (UniProt Id: P31648)
Reactivity	Reacts with: rat (P23978), mouse (P31648). Other species not tested yet.
Specificity	K.O. validated PubMed: 33664860
Matching control protein/peptide	274-1P
Remarks	WB: GABA transporter 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	274_102.pdf

References for GABA transporter1 - 274 102

Disrupting stroke-induced GAT-1-syntaxin1A interaction promotes functional recovery after stroke.
Lin YH, Wu F, Li TY, Lin L, Gao F, Zhu LJ, Xu XM, Chen MY, Hou YL, Zhang CJ, Wu HY, et al.
Cell reports. Medicine (2024) 511: 101789. 274 102 WB, ICC; tested species: mouse

GABA uptake transporters support dopamine release in dorsal striatum with maladaptive downregulation in a parkinsonism model.
Roberts BM, Doig NM, Brimblecombe KR, Lopes EF, Siddorn RE, Threlfell S, Connor-Robson N, Bengoa-Vergniory N, Pasternack N, Wade-Martins R, Magill PJ, et al.
Nature communications (2020) 111: 4958. 274 102 WB, IHC; tested species: mouse

Novel full-thickness biomimetic corneal model for studying pathogenesis and treatment of diabetic keratopathy.
Cui Z, Li X, Ou Y, Sun X, Gu J, Ding C, Yu Z, Guo Y, Liang Y, Mao S, Ma JH, et al.
Materials today. Bio (2025) 30: 101409. 274 102 ICC; tested species: human

A GABAergic system in atrioventricular node pacemaker cells controls electrical conduction between the atria and ventricles.
Liang D, Zhou L, Zhou H, Zhang F, Fang G, Leng J, Wu Y, Zhang Y, Yang A, Liu Y, Chen YH, et al.
Cell research (2024) : . 274 102 ICC, IHC; tested species: mouse

Gamma-Aminobutyric Acid Transporters in the Nucleus Tractus Solitarii Regulate Inhibitory and Excitatory Synaptic Currents That Influence Cardiorespiratory Function.
Martinez D, Lima-Silveira L, Matott MP, Hasser EM, Kline DD
Frontiers in physiology (2021) 12: 821110. 274 102 IHC; tested species: rat

Environmental enrichment implies GAT-1 as a potential therapeutic target for stroke recovery.
Lin Y, Yao M, Wu H, Wu F, Cao S, Ni H, Dong J, Yang D, Sun Y, Kou X, Li J, et al.
Theranostics (2021) 118: 3760-3780. 274 102 IHC; KO verified; tested species: mouse

Structural Heterogeneity of the GABAergic Tripartite Synapse.
Brunskine C, Passlick S, Henneberger C
Cells (2022) 1119: . 274 102 ExM; tested species: mouse

Cat. No.: 274 102

Amount: 200 µl

Price: $365.00

Structural Heterogeneity of the GABAergic Tripartite Synapse.
Brunskine C, Passlick S, Henneberger C
Cells (2022) 1119: . 274 102 ExM; tested species: mouse

ICC

IHC

IHC-P

Background

γ-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. After the release of GABA from synaptic vesicles into the synaptic cleft during neurotransmission, GABA transporters (GATs) remove extracellular GABA by reuptake into the presynaptic terminal.
Three GABA transporters are described so far of which only GAT 1 and GAT 3 are expressed in the brain.

1 ) Hu J et al., Neuropharmacology (2008)
2 ) Quick MW et al., J. Biol. Chem. (2004)
3 ) Law RM et al., J. Biol. Chem. (2000)
4 ) Beckman ML et al., J. Neurosci. (1998)
5 ) Ikegaki N et al., Brain Res. Mol. Brain Res. (1994)
6 ) Jursky F et al., J. Exp. Biol. (1994)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

GABA transporter1 antibody - 274 102 K.O.

References for GABA transporter1 - 274 102