GASP1 antibody - 264 003

GASP1 is involved in the sorting of GPCRs

Rabbit polyclonal purified antibody

Cat. No.: 264 003

Amount: 50 µg

Price: $380.00

Cat. No. 264 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 64 to 440 from mouse GASP1 (UniProt Id: Q5U4C1)
Reactivity	Reacts with: rat (Q920R4), mouse (Q5U4C1). Other species not tested yet.
Data sheet	264_003.pdf

Cat. No.: 264 003

Amount: 50 µg

Price: $380.00

Background

G-protein-coupled receptor proteins (GPCRs) are a huge protein family in mammals that mediate the physiological action of many neurotransmitters and neuromodulaters.
GPCR levels on the cell surface can be regulated by endocytosis and subsequent recycling or degradation of the internalized proteins. The G-protein-coupled receptor-associated sorting proteins GASP 1 and 2 play major roles in the postendocytic sorting of GPCRs to the degradative pathway.

1 ) Kargl J et al., Br. J. Pharmacol. (2012)
2 ) Martini L et al., FASEB J. (2007)
3 ) Tappe-Theodor A et al., J. Neurosci. (2007)
4 ) Bartlett SE et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
5 ) Simonin F et al., J. Neurochem. (2004)
6 ) Whistler JL et al., Science (2002)

Protocols