Synaptic Systems

Cat. No.: 221 011

Amount: 100 µg

Price: $415.00

Cat. No. 221 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	50G10
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 23 to 612 from human GARP (UniProt Id: Q14392)
Reactivity	Reacts with: human (Q14392). Other species not tested yet.
Remarks	Skimmed milk is required for efficient blocking.
Data sheet	221_011.pdf

References for GARP - 221 011

GARP: a key receptor controlling FOXP3 in human regulatory T cells.
Probst-Kepper M, Geffers R, Kröger A, Viegas N, Erck C, Hecht HJ, Lünsdorf H, Roubin R, Moharregh-Khiabani D, Wagner K, Ocklenburg F, et al.
Journal of cellular and molecular medicine (2009) 139B: 3343-57. 221 011 WB

Monoclonal antibodies against GARP/TGF-β1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo.
Cuende J, Liénart S, Dedobbeleer O, van der Woning B, De Boeck G, Stockis J, Huygens C, Colau D, Somja J, Delvenne P, Hannon M, et al.
Science translational medicine (2015) 7284: 284ra56. 221 011

Cat. No.: 221 011

Amount: 100 µg

Price: $415.00

Background

Regulatory T-cells (T_reg-cells) are involved in the homeostasis of immune reactions and tolerance. These specialized T-cells suppress auto-reactive cells and inhibit autoimmunity. Development and function of T_reg-cells depend on the expression of the transcription factor FOX3P.
The orphan receptor GARP, also referred to as LRRC 32, is exclusively found on T_reg-cells upon stimulation with anti-CD3/IL-2 or anti-CD3/anti-CD28/IL-2. Additionally it has been shown, that ectopic GARP expression strongly induces FOXP3.

1 ) Pilat N et al., Am. J. Transplant. (2010)
2 ) Battaglia M et al., Eur. J. Immunol. (2009)
3 ) Wang R et al., PLoS ONE (2008)
4 ) Valencia X et al., Nat Clin Pract Rheumatol (2007)

Protocols

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GARP antibody - 221 011

References for GARP - 221 011